Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells

Inga Kaufhold; Sünja Osbahr; Kensuke Shima; Sebastian Marwitz; Kristina Rohmann; Daniel Drömann; Torsten Goldmann; Klaus Dalhoff; Jan Rupp

doi:10.1016/j.micinf.2017.07.002

Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells

Inga Kaufhold^*, Sünja Osbahr, Kensuke Shima, Sebastian Marwitz, Kristina Rohmann, Daniel Drömann, Torsten Goldmann, Klaus Dalhoff, Jan Rupp

^*Korrespondierende/r Autor/-in für diese Arbeit

12 Zitate (Scopus)

Abstract

Loss of epithelial barriers characterized by reduction of E-cadherin is a hallmark of chronic obstructive pulmonary disease (COPD). We investigated the effects of nontypeable Haemophilus influenzae (NTHi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of E-cadherin in host cells. NTHi infection decreased E-cadherin mRNA and protein-levels in lung epithelial cells. E-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (FGF2), the mammalian target of rapamycin (mTOR) and Slug. These data indicate that epithelial integrity and barrier function is disturbed by NTHi infection. Mainly, the destruction of cell–cell contacts is a prominent feature in NTHi infection.

Originalsprache	Englisch
Zeitschrift	Microbes and Infection
Jahrgang	19
Ausgabenummer	11
Seiten (von - bis)	560-566
Seitenumfang	7
ISSN	1286-4579
DOIs	https://doi.org/10.1016/j.micinf.2017.07.002
Publikationsstatus	Veröffentlicht - 01.11.2017

Fördermittel

We thank Anke Hellberg and Kristin Wischnat (Clinic of Infectious Diseases and Microbiology, University of Lübeck) for excellent technical support and Christine Klein and Victor Krajka (Institute of Neurogenetics, University of Lübeck) for assistance with confocal microscopy. This work was supported in part by the BMBF/German Center of Infection Research and German Center for Lung Research.

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SDG 3 – Gesundheit und Wohlergehen

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Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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10.1016/j.micinf.2017.07.002

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title = "Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells",

abstract = "Loss of epithelial barriers characterized by reduction of E-cadherin is a hallmark of chronic obstructive pulmonary disease (COPD). We investigated the effects of nontypeable Haemophilus influenzae (NTHi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of E-cadherin in host cells. NTHi infection decreased E-cadherin mRNA and protein-levels in lung epithelial cells. E-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (FGF2), the mammalian target of rapamycin (mTOR) and Slug. These data indicate that epithelial integrity and barrier function is disturbed by NTHi infection. Mainly, the destruction of cell–cell contacts is a prominent feature in NTHi infection.",

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T1 - Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells

AU - Kaufhold, Inga

AU - Osbahr, Sünja

AU - Shima, Kensuke

AU - Marwitz, Sebastian

AU - Rohmann, Kristina

AU - Drömann, Daniel

AU - Goldmann, Torsten

AU - Dalhoff, Klaus

AU - Rupp, Jan

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Loss of epithelial barriers characterized by reduction of E-cadherin is a hallmark of chronic obstructive pulmonary disease (COPD). We investigated the effects of nontypeable Haemophilus influenzae (NTHi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of E-cadherin in host cells. NTHi infection decreased E-cadherin mRNA and protein-levels in lung epithelial cells. E-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (FGF2), the mammalian target of rapamycin (mTOR) and Slug. These data indicate that epithelial integrity and barrier function is disturbed by NTHi infection. Mainly, the destruction of cell–cell contacts is a prominent feature in NTHi infection.

AB - Loss of epithelial barriers characterized by reduction of E-cadherin is a hallmark of chronic obstructive pulmonary disease (COPD). We investigated the effects of nontypeable Haemophilus influenzae (NTHi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of E-cadherin in host cells. NTHi infection decreased E-cadherin mRNA and protein-levels in lung epithelial cells. E-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (FGF2), the mammalian target of rapamycin (mTOR) and Slug. These data indicate that epithelial integrity and barrier function is disturbed by NTHi infection. Mainly, the destruction of cell–cell contacts is a prominent feature in NTHi infection.

UR - https://www.scopus.com/pages/publications/85027964181

U2 - 10.1016/j.micinf.2017.07.002

DO - 10.1016/j.micinf.2017.07.002

M3 - Journal articles

AN - SCOPUS:85027964181

SN - 1286-4579

VL - 19

SP - 560

EP - 566

JO - Microbes and Infection

JF - Microbes and Infection

IS - 11

ER -

Nontypeable Haemophilus influenzae (NTHi) directly interfere with the regulation of E-cadherin in lung epithelial cells

Abstract

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