New nucleotide analogues with enhanced signal properties

Dmitry Cherkasov*, Thorsten Biet, Englbert Bäuml, Walther Traut, Michael Lohoff

*Korrespondierende/r Autor/-in für diese Arbeit
8 Zitate (Scopus)

Abstract

We describe synthesis and testing of a novel type of dye-modified nucleotides which we call macromolecular nucleotides (m-Nucs). Macromolecular nucleotides comprise a nucleotide moiety, a macromolecular linear linker, and a large macromolecular ligand carrying multiple fluorescent dyes. With incorporation of the nucleotide moiety into the growing nucleic acid strand during enzymatic synthesis, the macromolecular ligand together with the coupled dyes is bound to the nucleic acid. By the use of this new class of modified nucleotides, signals from multiple dye molecules can be obtained after a single enzymatic incorporation event. The modified nucleotides are considered especially useful in the fields of nanobiotechnology, where signal stability and intensity is a limiting factor.

OriginalspracheEnglisch
ZeitschriftBioconjugate Chemistry
Jahrgang21
Ausgabenummer1
Seiten (von - bis)122-129
Seitenumfang8
ISSN1043-1802
DOIs
PublikationsstatusVeröffentlicht - 20.01.2010

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