Neutrophils synthesize and activate TGFβ2

Christof H. Szymkowiak; Elena Csernok; Dirk Reinhold; Ute Bank; Wolfgang L. Gross; Jörn Kekow

doi:10.1006/cyto.1999.0572

Neutrophils synthesize and activate TGFβ2

Christof H. Szymkowiak, Elena Csernok, Dirk Reinhold, Ute Bank, Wolfgang L. Gross, Jörn Kekow^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Klinik für Rheumatologie und klinische Immunologie

Otto-von-Guericke-Universität Magdeburg

13 Zitate (Scopus)

Abstract

Human polymorphonuclear neutrophils (PMN) are regulated by soluble factors such as chemotactic peptides and cytokines. PMN themselves are capable of synthesizing and releasing various cytokines, thereby taking part in the afferent or inductive limb of the immune response. We report for the first time that TGFβ2 can be synthesized and released by PMN obtained from peripheral blood. Reverse transcriptase polymerase chain reaction (RT-PCR) showed that preparations of PMN express mRNA for transforming growth factor (TGF)β1 and TGFβ2 but not for TGFβ3. Only translation of TGFβ2 into protein was observed applying different assays. Interestingly, TGFβ2 in supernatants from stimulated PMN could be detected in the bioactive form. (C) 2000 Academic Press.

Originalsprache	Englisch
Zeitschrift	Cytokine
Jahrgang	12
Ausgabenummer	4
Seiten (von - bis)	397-400
Seitenumfang	4
ISSN	1043-4666
DOIs	https://doi.org/10.1006/cyto.1999.0572
Publikationsstatus	Veröffentlicht - 04.2000

Fördermittel

The author would like to thank I. Mons and K. Bartels for excellent technical assistance. This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 387), Bonn, Germany.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

Dokumente

10.1006/cyto.1999.0572

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title = "Neutrophils synthesize and activate TGFβ2",

abstract = "Human polymorphonuclear neutrophils (PMN) are regulated by soluble factors such as chemotactic peptides and cytokines. PMN themselves are capable of synthesizing and releasing various cytokines, thereby taking part in the afferent or inductive limb of the immune response. We report for the first time that TGFβ2 can be synthesized and released by PMN obtained from peripheral blood. Reverse transcriptase polymerase chain reaction (RT-PCR) showed that preparations of PMN express mRNA for transforming growth factor (TGF)β1 and TGFβ2 but not for TGFβ3. Only translation of TGFβ2 into protein was observed applying different assays. Interestingly, TGFβ2 in supernatants from stimulated PMN could be detected in the bioactive form. (C) 2000 Academic Press.",

author = "Szymkowiak, \{Christof H.\} and Elena Csernok and Dirk Reinhold and Ute Bank and Gross, \{Wolfgang L.\} and J{\"o}rn Kekow",

note = "Funding Information: The author would like to thank I. Mons and K. Bartels for excellent technical assistance. This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 387), Bonn, Germany. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2000",

month = apr,

doi = "10.1006/cyto.1999.0572",

language = "English",

volume = "12",

pages = "397--400",

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T1 - Neutrophils synthesize and activate TGFβ2

AU - Szymkowiak, Christof H.

AU - Csernok, Elena

AU - Reinhold, Dirk

AU - Bank, Ute

AU - Gross, Wolfgang L.

AU - Kekow, Jörn

N1 - Funding Information: The author would like to thank I. Mons and K. Bartels for excellent technical assistance. This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 387), Bonn, Germany. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2000/4

Y1 - 2000/4

N2 - Human polymorphonuclear neutrophils (PMN) are regulated by soluble factors such as chemotactic peptides and cytokines. PMN themselves are capable of synthesizing and releasing various cytokines, thereby taking part in the afferent or inductive limb of the immune response. We report for the first time that TGFβ2 can be synthesized and released by PMN obtained from peripheral blood. Reverse transcriptase polymerase chain reaction (RT-PCR) showed that preparations of PMN express mRNA for transforming growth factor (TGF)β1 and TGFβ2 but not for TGFβ3. Only translation of TGFβ2 into protein was observed applying different assays. Interestingly, TGFβ2 in supernatants from stimulated PMN could be detected in the bioactive form. (C) 2000 Academic Press.

AB - Human polymorphonuclear neutrophils (PMN) are regulated by soluble factors such as chemotactic peptides and cytokines. PMN themselves are capable of synthesizing and releasing various cytokines, thereby taking part in the afferent or inductive limb of the immune response. We report for the first time that TGFβ2 can be synthesized and released by PMN obtained from peripheral blood. Reverse transcriptase polymerase chain reaction (RT-PCR) showed that preparations of PMN express mRNA for transforming growth factor (TGF)β1 and TGFβ2 but not for TGFβ3. Only translation of TGFβ2 into protein was observed applying different assays. Interestingly, TGFβ2 in supernatants from stimulated PMN could be detected in the bioactive form. (C) 2000 Academic Press.

UR - https://www.scopus.com/pages/publications/0033923151

U2 - 10.1006/cyto.1999.0572

DO - 10.1006/cyto.1999.0572

M3 - Journal articles

C2 - 10805222

AN - SCOPUS:0033923151

SN - 1043-4666

VL - 12

SP - 397

EP - 400

JO - Cytokine

JF - Cytokine

IS - 4

ER -

Neutrophils synthesize and activate TGFβ2

Abstract

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