Naive, effector, and memory T lymphocytes efficiently scan dendritic cells in vivo: Contact frequency in T cell zones of secondary lymphoid organs does not depend on LFA-1 expression and facilitates survival of effector T cells

Jürgen Westermann*, Ulrike Bode, Andrea Sahle, Uwe Speck, Nathan Karin, Eric B. Bell, Kathrin Kalies, Andreas Gebert

*Korrespondierende/r Autor/-in für diese Arbeit
27 Zitate (Scopus)

Abstract

Contact between T cells and dendritic cells (DCs) is required for their subsequent interaction leading to the induction of adaptive immune responses. Quantitative data regarding the contact frequencies of T cell subsets in different lymphoid organs and species are lacking. Therefore, naive, effector, and memory CD4 T cells were injected into rats in absence of the cognate Ag, and 0.5-96 h later, spleen, lymph nodes, and Peyer's patches were removed. Cryosections were analyzed for contact between donor T cells and endogenous DCs in the T cell zone, and donor cell proliferation. More than 60% of injected naive CD4 T cells were in contact with endogenous DCs at all time points and in all organs analyzed. Surprisingly, we were unable to detect any differences between naive, effector, and memory CD4 T cells despite different expression levels of surface molecules. In addition, contact frequency was similar for T cells in lymphoid organs of rats, mice, and humans; it was unaffected by the absence of LFA-1 (CD11a/CD18), and sustained effector T cells in an activated state. Thus, the architecture of the T cell zone ratlier than expression patterns of surface molecules determines the contact efficiency between T cells and DCs in vivo.

OriginalspracheEnglisch
ZeitschriftJournal of Immunology
Jahrgang174
Ausgabenummer5
Seiten (von - bis)2517-2524
Seitenumfang8
ISSN0022-1767
DOIs
PublikationsstatusVeröffentlicht - 01.03.2005

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)

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