Murine norovirus-1 entry into permissive macrophages and dendritic cells is pH-independent

Jeffrey W. Perry; Stefan Taube; Christiane E. Wobus

doi:10.1016/j.virusres.2009.03.002

Murine norovirus-1 entry into permissive macrophages and dendritic cells is pH-independent

Jeffrey W. Perry, Stefan Taube, Christiane E. Wobus^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Virologie und Zellbiologie

University of Michigan

38 Zitate (Scopus)

Abstract

Murine norovirus (MNV) is a recently discovered mouse pathogen. Unlike the fastidious human noroviruses that cause the overwhelming majority of non-bacterial gastroenteritis worldwide, MNV readily infects cells in culture. Its replication in primary murine macrophages and dendritic cells and their derived cell lines allows the study of norovirus cell entry for the first time. In this study we determined the role of pH during MNV-1 infection since the low pH environment of endosomes often triggers uncoating of viruses. We demonstrated that MNV-1 viral titers by plaque assay and expression of the non-structural protein VPg by immunofluorescence were not affected by pH in cultured and primary macrophages and dendritic cells in the presence of two known endosome acidification inhibitors, bafilomycin A1 and chloroquine. These data indicate that MNV-1 enters permissive cells in a pH-independent manner.

Originalsprache	Englisch
Zeitschrift	Virus Research
Jahrgang	143
Ausgabenummer	1
Seiten (von - bis)	125-129
Seitenumfang	5
ISSN	0168-1702
DOIs	https://doi.org/10.1016/j.virusres.2009.03.002
Publikationsstatus	Veröffentlicht - 01.07.2009

Fördermittel

This work was sponsored in part by the NIH/NIAID Regional Center of Excellence for Bio-defense and Emerging Infectious Diseases Research (RCE) Program, Region V ‘Great Lakes’ RCE (NIH award 1-U54-AI-057153) to C.W. J.P. was funded by the University of Michigan Human Genetics Training Grant (grant NIH T32 GM 07544). We would like to thank Drs. Kim Green (NIAID, NIH, USA), Ian Clark (University of Southampton, UK), and John Connor (Boston University, USA) for their generous gifts of antibodies, Dr. Isabelle Dimier-Poisson (University of Tours, France) for the SRDC cell line, and members of the Center for Statistical Consultation and Research at the University of Michigan for advice on statistical analysis. We are indebted to Drs. Kathy Spindler and Oveta Fuller (University of Michigan, USA) for critical reading of the manuscript and helpful suggestions.

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10.1016/j.virusres.2009.03.002

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title = "Murine norovirus-1 entry into permissive macrophages and dendritic cells is pH-independent",

abstract = "Murine norovirus (MNV) is a recently discovered mouse pathogen. Unlike the fastidious human noroviruses that cause the overwhelming majority of non-bacterial gastroenteritis worldwide, MNV readily infects cells in culture. Its replication in primary murine macrophages and dendritic cells and their derived cell lines allows the study of norovirus cell entry for the first time. In this study we determined the role of pH during MNV-1 infection since the low pH environment of endosomes often triggers uncoating of viruses. We demonstrated that MNV-1 viral titers by plaque assay and expression of the non-structural protein VPg by immunofluorescence were not affected by pH in cultured and primary macrophages and dendritic cells in the presence of two known endosome acidification inhibitors, bafilomycin A1 and chloroquine. These data indicate that MNV-1 enters permissive cells in a pH-independent manner.",

author = "Perry, \{Jeffrey W.\} and Stefan Taube and Wobus, \{Christiane E.\}",

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T1 - Murine norovirus-1 entry into permissive macrophages and dendritic cells is pH-independent

AU - Perry, Jeffrey W.

AU - Taube, Stefan

AU - Wobus, Christiane E.

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Y1 - 2009/7/1

N2 - Murine norovirus (MNV) is a recently discovered mouse pathogen. Unlike the fastidious human noroviruses that cause the overwhelming majority of non-bacterial gastroenteritis worldwide, MNV readily infects cells in culture. Its replication in primary murine macrophages and dendritic cells and their derived cell lines allows the study of norovirus cell entry for the first time. In this study we determined the role of pH during MNV-1 infection since the low pH environment of endosomes often triggers uncoating of viruses. We demonstrated that MNV-1 viral titers by plaque assay and expression of the non-structural protein VPg by immunofluorescence were not affected by pH in cultured and primary macrophages and dendritic cells in the presence of two known endosome acidification inhibitors, bafilomycin A1 and chloroquine. These data indicate that MNV-1 enters permissive cells in a pH-independent manner.

AB - Murine norovirus (MNV) is a recently discovered mouse pathogen. Unlike the fastidious human noroviruses that cause the overwhelming majority of non-bacterial gastroenteritis worldwide, MNV readily infects cells in culture. Its replication in primary murine macrophages and dendritic cells and their derived cell lines allows the study of norovirus cell entry for the first time. In this study we determined the role of pH during MNV-1 infection since the low pH environment of endosomes often triggers uncoating of viruses. We demonstrated that MNV-1 viral titers by plaque assay and expression of the non-structural protein VPg by immunofluorescence were not affected by pH in cultured and primary macrophages and dendritic cells in the presence of two known endosome acidification inhibitors, bafilomycin A1 and chloroquine. These data indicate that MNV-1 enters permissive cells in a pH-independent manner.

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DO - 10.1016/j.virusres.2009.03.002

M3 - Journal articles

C2 - 19463729

AN - SCOPUS:67349267018

SN - 0168-1702

VL - 143

SP - 125

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Murine norovirus-1 entry into permissive macrophages and dendritic cells is pH-independent

Abstract

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