Monitoring changing cellular characteristics during the development of a fin cell line from Cyprinus carpio

Sebastian Rakers*, Anne Kathrin Ondrusch, Martina Gruening, Mikolaj Adamek, Benita Moeckel, Marina Gebert

*Korrespondierende/r Autor/-in für diese Arbeit

Abstract

The establishment and in-depth characterization of a novel continuous cell line derived from fin tissue of common carp (Cyprinus carpio), CCApin, is reported. The cells of the cell line could be propagated in Leibovitz's L-15 medium containing 15% foetal calf serum and 0.5% carp serum for >150 passages during the last 24 months, with a stable fast growth. Furthermore, antibody staining indicated that cell types obtained in primary cultures, containing the epithelial stem-cell marker tumorprotein 63, were different from cells in long-term cell cultures, containing tight junction protein zona occludens 1 and cytokeratin 7. These observations suggest a switch of dominant cell types. Molecular analysis of gene expression profiles of caudal fin tissue and CCApin cells showed that genes relevant in epithelial cells but also in mesenchymal cells were expressed. However, during cultivation of CCApin a set of very steadily expressed, primarily mesenchymal genes like collagen 1 alpha 1, fibronectin or cadherin 2 was found. In summary, the long-term cell culture could be described as a stably growing epithelial population with some mesenchymal features. There are several application possibilities, especially for virus susceptibility studies, e.g. cyprinid herpesvirus-3 (CyHV-3). The study leads to a better understanding of molecular and physiological mechanisms of in vitro fish cell cultures.

OriginalspracheEnglisch
ZeitschriftComparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Jahrgang225
Seiten (von - bis)1-12
Seitenumfang12
ISSN1096-4959
DOIs
PublikationsstatusVeröffentlicht - 01.11.2018
Extern publiziertJa

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Biomedizintechnik

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