Metabolomic analysis of diapausing and noni-diapausing larvae of the European corn borer Ostrinia nubilalis (Hbn.) (Lepidoptera Crambidae)

Jelena Purać; Danijela Kojić; Željko D. Popović; Elvira Vukašinović; Stefano Tiziani; Ulrich L. Günther; Gordana Grubor-Lajšić

doi:10.17344/acsi.2015.1370

Metabolomic analysis of diapausing and noni-diapausing larvae of the European corn borer Ostrinia nubilalis (Hbn.) (Lepidoptera Crambidae)

Jelena Purać^*, Danijela Kojić, Željko D. Popović, Elvira Vukašinović, Stefano Tiziani, Ulrich L. Günther, Gordana Grubor-Lajšić

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Chemie und Metabolomics

13 Zitate (Scopus)

Abstract

In this study, an ¹H-NMR-based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to -3 °C and -16 °C, alanine, glycerol and acetate were predominant metabolites. ¹H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.

Originalsprache	Englisch
Zeitschrift	Acta Chimica Slovenica
Jahrgang	62
Ausgabenummer	4
Seiten (von - bis)	761-767
Seitenumfang	7
ISSN	1318-0207
DOIs	https://doi.org/10.17344/acsi.2015.1370
Publikationsstatus	Veröffentlicht - 2015

Fördermittel

This work was funded by the Ministry of Science and Technological Development, Republic of Serbia, grant 173014 "Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology". The authors thank the Wellcome Trust and the EU in the context of the EU-NMR grant (RII3-026145) for supporting the HWBNMR facility in Birmingham. The authors thank the EU for supporting S.T. in the context of the MOTET Marie Curie project. We thank Dr. Edward Petri for editing this manuscript for English grammar and content.

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

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10.17344/acsi.2015.1370

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@article{b9c8c1990a6f41b2a2289c059febd146,

title = "Metabolomic analysis of diapausing and noni-diapausing larvae of the European corn borer Ostrinia nubilalis (Hbn.) (Lepidoptera Crambidae)",

abstract = "In this study, an 1H-NMR-based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to -3 °C and -16 °C, alanine, glycerol and acetate were predominant metabolites. 1H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.",

author = "Jelena Pura{\'c} and Danijela Koji{\'c} and Popovi{\'c}, \{{\v Z}eljko D.\} and Elvira Vuka{\v s}inovi{\'c} and Stefano Tiziani and G{\"u}nther, \{Ulrich L.\} and Gordana Grubor-Laj{\v s}i{\'c}",

note = "Funding Information: This work was funded by the Ministry of Science and Technological Development, Republic of Serbia, grant 173014 {"}Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology{"}. The authors thank the Wellcome Trust and the EU in the context of the EU-NMR grant (RII3-026145) for supporting the HWBNMR facility in Birmingham. The authors thank the EU for supporting S.T. in the context of the MOTET Marie Curie project. We thank Dr. Edward Petri for editing this manuscript for English grammar and content. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.",

year = "2015",

doi = "10.17344/acsi.2015.1370",

language = "English",

volume = "62",

pages = "761--767",

journal = "Acta Chimica Slovenica",

issn = "1318-0207",

publisher = "Slovensko Kemijsko Drustvo",

number = "4",

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Metabolomic analysis of diapausing and noni-diapausing larvae of the European corn borer Ostrinia nubilalis (Hbn.) (Lepidoptera Crambidae). / Purać, Jelena; Kojić, Danijela; Popović, Željko D. et al.
in: Acta Chimica Slovenica, Jahrgang 62, Nr. 4, 2015, S. 761-767.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - Metabolomic analysis of diapausing and noni-diapausing larvae of the European corn borer Ostrinia nubilalis (Hbn.) (Lepidoptera Crambidae)

AU - Purać, Jelena

AU - Kojić, Danijela

AU - Popović, Željko D.

AU - Vukašinović, Elvira

AU - Tiziani, Stefano

AU - Günther, Ulrich L.

AU - Grubor-Lajšić, Gordana

N1 - Funding Information: This work was funded by the Ministry of Science and Technological Development, Republic of Serbia, grant 173014 "Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology". The authors thank the Wellcome Trust and the EU in the context of the EU-NMR grant (RII3-026145) for supporting the HWBNMR facility in Birmingham. The authors thank the EU for supporting S.T. in the context of the MOTET Marie Curie project. We thank Dr. Edward Petri for editing this manuscript for English grammar and content. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.

PY - 2015

Y1 - 2015

N2 - In this study, an 1H-NMR-based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to -3 °C and -16 °C, alanine, glycerol and acetate were predominant metabolites. 1H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.

AB - In this study, an 1H-NMR-based metabolomic approach was used to investigate the biochemical mechanisms of diapause and cold hardiness in diapausing larvae of the European corn borer Ostrinia nubilalis. Metabolomic patterns in polar hemolymph extracts from non-diapausing and diapausing larvae of O. nubilalis were compared. Analysis indicated 13 metabolites: 7 amino acids, glycerol, acetate, citrate, succinate, lactate and putrescine. Results show that diapausing larvae display different metabolomic patterns compared to active non-diapausing larvae, with predominant metabolites identified as glycerol, proline and alanine. In specific diapausing larvae initially kept at 5 °C then gradually chilled to -3 °C and -16 °C, alanine, glycerol and acetate were predominant metabolites. 1H-NMR spectroscopy provides new insight into the metabolomic patterns associated with cold resistance and diapause in O. nubilalis larvae, suggesting distinct metabolomes function in actively developing and diapausing larvae.

UR - https://www.scopus.com/pages/publications/84954149211

U2 - 10.17344/acsi.2015.1370

DO - 10.17344/acsi.2015.1370

M3 - Journal articles

C2 - 26680702

AN - SCOPUS:84954149211

SN - 1318-0207

VL - 62

SP - 761

EP - 767

JO - Acta Chimica Slovenica

JF - Acta Chimica Slovenica

IS - 4

ER -

Metabolomic analysis of diapausing and noni-diapausing larvae of the European corn borer Ostrinia nubilalis (Hbn.) (Lepidoptera Crambidae)

Abstract

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