Low temperature study of myoglobin-ligand rebinding kinetics with Mossbauer spectroscopy

C. Ober, M. Burkardt, H. Winkler, A. X. Trautwein*, A. A. Zharikov, S. F. Fischer, F. Parak

*Korrespondierende/r Autor/-in für diese Arbeit
5 Zitate (Scopus)

Abstract

We have studied the recombination kinetics of carboxymyoglobin (after photodissociation of the CO ligand) by Mossbauer spectroscopy for temperatures in the range 4.2-60 K. The observed kinetics display non-exponential behaviour which was monitored over periods of a few days. It is shown that the time dependence of the kinetics can be reduced to a single universal function of the temperature-dependent variable (t/τ(1/2)(T))(β(T)). The half-decay time τ(1/2)(T) and the scaling parameter β(T) are analysed for the presence of tunneling effects. The non-Arrhenius temperature dependence of the half-decay time below 60 K is interpreted as activated tunneling in models with an Eckart barrier or a fluctuating barrier.

OriginalspracheEnglisch
ZeitschriftEuropean Biophysics Journal
Jahrgang26
Ausgabenummer3
Seiten (von - bis)227-237
Seitenumfang11
ISSN0175-7571
DOIs
PublikationsstatusVeröffentlicht - 01.01.1997

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