Live Calcium Imaging on Mouse Primary Brain Endothelial Cells

Dimitrios Spyropoulos; Jan Wenzel

doi:10.1007/978-1-0716-4706-6_11

Live Calcium Imaging on Mouse Primary Brain Endothelial Cells

Dimitrios Spyropoulos, Jan Wenzel

Institut für Experimentelle und Klinische Pharmakologie und Toxikologie

1 Zitat (Scopus)

Abstract

Calcium ions (Ca2+) are crucial as universal second messenger signaling molecules in numerous cellular processes. In the cardiovascular system, intracellular calcium signaling is important for regulating vascular resistance, functional hyperemia, leukocyte transmigration, and coagulation. In vitro and in vivo live calcium imaging is a pivotal technique in modern cellular biology, allowing the visualization of intracellular calcium dynamics with high temporal and spatial resolution. Calcium imaging is particularly significant for studying the downstream effects of ion channels, G protein-coupled receptors (GPCRs), and other signaling pathways that modulate cellular calcium levels. Here, we describe a simple and fast method of investigating the intracellular calcium changes in an in vitro blood-brain barrier model by utilizing the fluorescent dye Fluo-4 AM in primary brain endothelial cells.

Originalsprache	Englisch
Zeitschrift	Methods in molecular biology (Clifton, N.J.)
Jahrgang	2956
Seiten (von - bis)	119-124
Seitenumfang	6
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-0716-4706-6_11
Publikationsstatus	Veröffentlicht - 2025

Fördermittel

The research leading to these results received funding from European Union’s Horizon 2020 research and innovation progrunder the Marie Skłodowska-Curie grant agreement (ENTRAINo. 813294 and from the Deutsche Forschungsgemeinsch(WE 6456/1-1) to J. Wenzel.

Träger	Trägernummer
H2020 Marie Skłodowska-Curie Actions	813294
Deutsche Forschungsgemeinschaft	WE 6456/1-1

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Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

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Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)

DFG-Fachsystematik

2.23-05 Experimentelle Modelle zum Verständnis von Erkrankungen des Nervensystems

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10.1007/978-1-0716-4706-6_11

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title = "Live Calcium Imaging on Mouse Primary Brain Endothelial Cells",

abstract = "Calcium ions (Ca2+) are crucial as universal second messenger signaling molecules in numerous cellular processes. In the cardiovascular system, intracellular calcium signaling is important for regulating vascular resistance, functional hyperemia, leukocyte transmigration, and coagulation. In vitro and in vivo live calcium imaging is a pivotal technique in modern cellular biology, allowing the visualization of intracellular calcium dynamics with high temporal and spatial resolution. Calcium imaging is particularly significant for studying the downstream effects of ion channels, G protein-coupled receptors (GPCRs), and other signaling pathways that modulate cellular calcium levels. Here, we describe a simple and fast method of investigating the intracellular calcium changes in an in vitro blood-brain barrier model by utilizing the fluorescent dye Fluo-4 AM in primary brain endothelial cells.",

author = "Dimitrios Spyropoulos and Jan Wenzel",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",

year = "2025",

doi = "10.1007/978-1-0716-4706-6\_11",

language = "English",

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T1 - Live Calcium Imaging on Mouse Primary Brain Endothelial Cells

AU - Spyropoulos, Dimitrios

AU - Wenzel, Jan

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.

PY - 2025

Y1 - 2025

N2 - Calcium ions (Ca2+) are crucial as universal second messenger signaling molecules in numerous cellular processes. In the cardiovascular system, intracellular calcium signaling is important for regulating vascular resistance, functional hyperemia, leukocyte transmigration, and coagulation. In vitro and in vivo live calcium imaging is a pivotal technique in modern cellular biology, allowing the visualization of intracellular calcium dynamics with high temporal and spatial resolution. Calcium imaging is particularly significant for studying the downstream effects of ion channels, G protein-coupled receptors (GPCRs), and other signaling pathways that modulate cellular calcium levels. Here, we describe a simple and fast method of investigating the intracellular calcium changes in an in vitro blood-brain barrier model by utilizing the fluorescent dye Fluo-4 AM in primary brain endothelial cells.

AB - Calcium ions (Ca2+) are crucial as universal second messenger signaling molecules in numerous cellular processes. In the cardiovascular system, intracellular calcium signaling is important for regulating vascular resistance, functional hyperemia, leukocyte transmigration, and coagulation. In vitro and in vivo live calcium imaging is a pivotal technique in modern cellular biology, allowing the visualization of intracellular calcium dynamics with high temporal and spatial resolution. Calcium imaging is particularly significant for studying the downstream effects of ion channels, G protein-coupled receptors (GPCRs), and other signaling pathways that modulate cellular calcium levels. Here, we describe a simple and fast method of investigating the intracellular calcium changes in an in vitro blood-brain barrier model by utilizing the fluorescent dye Fluo-4 AM in primary brain endothelial cells.

UR - https://www.scopus.com/pages/publications/105015239432

U2 - 10.1007/978-1-0716-4706-6_11

DO - 10.1007/978-1-0716-4706-6_11

M3 - Journal articles

C2 - 40859080

SN - 1064-3745

VL - 2956

SP - 119

EP - 124

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Live Calcium Imaging on Mouse Primary Brain Endothelial Cells

Abstract

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