Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue

Sazgar Burhan; Madita Göb; Mario Pieper; Tjalfe Laedtke; Thorge Grahl; Michael Münter; Hinnerk Schulz-Hildebrandt; Gereon Hüttmann; Peter König; Robert Huber

doi:10.1117/12.3046216

Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue

Sazgar Burhan, Madita Göb, Mario Pieper, Tjalfe Laedtke, Thorge Grahl, Michael Münter, Hinnerk Schulz-Hildebrandt, Gereon Hüttmann, Peter König, Robert Huber

Abstract

We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm². Validations against histological sections confirmed the system’s ability to visualize cellular tissue structures.

Originalsprache	Englisch
Titel	Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX
Redakteure/-innen	Rainer A. Leitgeb, Yoshiaki Yasuno
Seitenumfang	1
Band	13305
Herausgeber (Verlag)	SPIE
Erscheinungsdatum	03.2025
Seiten	1330502
DOIs	https://doi.org/10.1117/12.3046216
Publikationsstatus	Veröffentlicht - 03.2025

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10.1117/12.3046216

https://doi.org/10.1117/12.3046216

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Burhan, S., Göb, M., Pieper, M., Laedtke, T., Grahl, T., Münter, M., Schulz-Hildebrandt, H., Hüttmann, G., König, P., & Huber, R. (2025). Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue. in R. A. Leitgeb, & Y. Yasuno (Hrsg.), Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX (Band 13305, S. 1330502). SPIE. https://doi.org/10.1117/12.3046216

@inproceedings{cbdee7dc33114844bd13531a1172e423,

title = "Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue",

abstract = "We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm2. Validations against histological sections confirmed the system{\textquoteright}s ability to visualize cellular tissue structures.",

author = "Sazgar Burhan and Madita G{\"o}b and Mario Pieper and Tjalfe Laedtke and Thorge Grahl and Michael M{\"u}nter and Hinnerk Schulz-Hildebrandt and Gereon H{\"u}ttmann and Peter K{\"o}nig and Robert Huber",

year = "2025",

month = mar,

doi = "10.1117/12.3046216",

language = "English",

volume = "13305",

pages = "1330502",

editor = "Leitgeb, {Rainer A.} and Yoshiaki Yasuno",

booktitle = "Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX",

publisher = "SPIE",

}

Burhan, S , Göb, M , Pieper, M, Laedtke, T, Grahl, T, Münter, M, Schulz-Hildebrandt, H, Hüttmann, G , König, P & Huber, R 2025, Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue. in RA Leitgeb & Y Yasuno (Hrsg.), Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX. Bd. 13305, SPIE, S. 1330502. https://doi.org/10.1117/12.3046216

Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue. / Burhan, Sazgar ; Göb, Madita ; Pieper, Mario et al.
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX. Hrsg. / Rainer A. Leitgeb; Yoshiaki Yasuno. Band 13305 SPIE, 2025. S. 1330502.

Publikation: Kapitel in Büchern/Berichten/Konferenzbänden › Konferenzbeitrag › Begutachtung

TY - GEN

T1 - Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue

AU - Burhan, Sazgar

AU - Göb, Madita

AU - Pieper, Mario

AU - Laedtke, Tjalfe

AU - Grahl, Thorge

AU - Münter, Michael

AU - Schulz-Hildebrandt, Hinnerk

AU - Hüttmann, Gereon

AU - König, Peter

AU - Huber, Robert

PY - 2025/3

Y1 - 2025/3

N2 - We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm2. Validations against histological sections confirmed the system’s ability to visualize cellular tissue structures.

AB - We demonstrate a 3.2 MHz-OCT system for inter-volumetric dynamic optical coherence tomography of ex vivo porcine kidney tissue. Employing a home-built Fourier Domain mode locking (FDML) laser with a 1310 nm wavelength, the system achieved a lateral resolution of 3.48 μm and a frame rate of 612 Hz. A motorized XYZ positioning stage enabled the precise acquisition of multiple volumes, which were seamlessly stitched together to generate a comprehensive dataset with a total area of 2.6 × 2.6 mm2. Validations against histological sections confirmed the system’s ability to visualize cellular tissue structures.

U2 - 10.1117/12.3046216

DO - 10.1117/12.3046216

M3 - Conference contribution

VL - 13305

SP - 1330502

BT - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIX

A2 - Leitgeb, Rainer A.

A2 - Yasuno, Yoshiaki

PB - SPIE

ER -

Large-area dynamic contrast MHz optical coherence tomography for label-free imaging of porcine tissue

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