Isolation and characterization of novel CAG repeat containing genes expressed in human brain

Christine Zühlke; Rasmus Kiehl; Antje Johannsmeyer; Karl Heinz Grzeschik; Eberhard Schwinger

doi:10.3109/10425179909033929

Isolation and characterization of novel CAG repeat containing genes expressed in human brain

Christine Zühlke^*, Rasmus Kiehl, Antje Johannsmeyer, Karl Heinz Grzeschik, Eberhard Schwinger

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Humangenetik

Philipps-Universität Marburg

5 Zitate (Scopus)

Abstract

Neurodegenerative diseases may be caused by expansion of triplet repeats in human genes. To identify novel genes with CAG repeats, we screened a human brain cDNA library with an oligonucleotide probe. Four of the isolated cDNAs were sequenced, analyzed for polymorphisms, chromosomal localization, evolutionary conservation and expression. One of the repeats is bi-allelic with 10 triplets (80 % of chromosomes) and 7 triplets (20 % of chromosomes). In one of the genes two CAG repeats coding for 10 and 17 glutamines are localized in the same reading frame.

Originalsprache	Englisch
Zeitschrift	Mitochondrial DNA
Jahrgang	10
Ausgabenummer	1
Seiten (von - bis)	1-6
Seitenumfang	6
ISSN	1940-1736
DOIs	https://doi.org/10.3109/10425179909033929
Publikationsstatus	Veröffentlicht - 1999

Fördermittel

This work was supported by the Forschungs-forderungsprogramm der Medizinischen Uni- versitat Lubeck (89/96). Ch. Z. wish to thank Prof. Dr. W. Engel (Gottingen) for continous support, Dr. 0. Riess (Bochum) for helpful discussions, and S. Herlt, K. Schulz, S. Wagner, Institut fiir Humangenetik in Gottingen, J. Leu-telt and H. Bottger, Institut fur Humangenetik in Lubeck, for excellent technical assistance.

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10.3109/10425179909033929

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title = "Isolation and characterization of novel CAG repeat containing genes expressed in human brain",

abstract = "Neurodegenerative diseases may be caused by expansion of triplet repeats in human genes. To identify novel genes with CAG repeats, we screened a human brain cDNA library with an oligonucleotide probe. Four of the isolated cDNAs were sequenced, analyzed for polymorphisms, chromosomal localization, evolutionary conservation and expression. One of the repeats is bi-allelic with 10 triplets (80 \% of chromosomes) and 7 triplets (20 \% of chromosomes). In one of the genes two CAG repeats coding for 10 and 17 glutamines are localized in the same reading frame.",

author = "Christine Z{\"u}hlke and Rasmus Kiehl and Antje Johannsmeyer and Grzeschik, \{Karl Heinz\} and Eberhard Schwinger",

note = "Funding Information: This work was supported by the Forschungs-forderungsprogramm der Medizinischen Uni- versitat Lubeck (89/96). Ch. Z. wish to thank Prof. Dr. W. Engel (Gottingen) for continous support, Dr. 0. Riess (Bochum) for helpful discussions, and S. Herlt, K. Schulz, S. Wagner, Institut fiir Humangenetik in Gottingen, J. Leu-telt and H. Bottger, Institut fur Humangenetik in Lubeck, for excellent technical assistance.",

year = "1999",

doi = "10.3109/10425179909033929",

language = "English",

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AU - Zühlke, Christine

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AU - Johannsmeyer, Antje

AU - Grzeschik, Karl Heinz

AU - Schwinger, Eberhard

N1 - Funding Information: This work was supported by the Forschungs-forderungsprogramm der Medizinischen Uni- versitat Lubeck (89/96). Ch. Z. wish to thank Prof. Dr. W. Engel (Gottingen) for continous support, Dr. 0. Riess (Bochum) for helpful discussions, and S. Herlt, K. Schulz, S. Wagner, Institut fiir Humangenetik in Gottingen, J. Leu-telt and H. Bottger, Institut fur Humangenetik in Lubeck, for excellent technical assistance.

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N2 - Neurodegenerative diseases may be caused by expansion of triplet repeats in human genes. To identify novel genes with CAG repeats, we screened a human brain cDNA library with an oligonucleotide probe. Four of the isolated cDNAs were sequenced, analyzed for polymorphisms, chromosomal localization, evolutionary conservation and expression. One of the repeats is bi-allelic with 10 triplets (80 % of chromosomes) and 7 triplets (20 % of chromosomes). In one of the genes two CAG repeats coding for 10 and 17 glutamines are localized in the same reading frame.

AB - Neurodegenerative diseases may be caused by expansion of triplet repeats in human genes. To identify novel genes with CAG repeats, we screened a human brain cDNA library with an oligonucleotide probe. Four of the isolated cDNAs were sequenced, analyzed for polymorphisms, chromosomal localization, evolutionary conservation and expression. One of the repeats is bi-allelic with 10 triplets (80 % of chromosomes) and 7 triplets (20 % of chromosomes). In one of the genes two CAG repeats coding for 10 and 17 glutamines are localized in the same reading frame.

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DO - 10.3109/10425179909033929

M3 - Journal articles

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AN - SCOPUS:0344223299

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VL - 10

SP - 1

EP - 6

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JF - Mitochondrial DNA

IS - 1

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Isolation and characterization of novel CAG repeat containing genes expressed in human brain

Abstract

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