Investigation of laser-induced cell lysis using time-resolved imaging

Kaustubh R. Rau; Arnold Guerra; Alfred Vogel; Vasan Venugopalan

doi:10.1063/1.1705728

Investigation of laser-induced cell lysis using time-resolved imaging

Kaustubh R. Rau
, Arnold Guerra
, Alfred Vogel
, Vasan Venugopalan^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Biomedizinische Optik

University of California, Irvine

101 Zitate (Scopus)

Abstract

The lysis of confluent PtK2 cell cultures was investigated by pulsed laser microbeam irradiation. Lysis to be mediated by laser-induced plasma formation was demonstrated by images obtained at time delays of 0.5 ns to 50 μs. The quantitative characterization of the pressure wave and cavitation bubble dynamics were enabled by image analysis. The results show that the zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansions as the primary agent of cell injury.

Originalsprache	Englisch
Zeitschrift	Applied Physics Letters
Jahrgang	84
Ausgabenummer	15
Seiten (von - bis)	2940-2942
Seitenumfang	3
ISSN	0003-6951
DOIs	https://doi.org/10.1063/1.1705728
Publikationsstatus	Veröffentlicht - 12.04.2004

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abstract = "The lysis of confluent PtK2 cell cultures was investigated by pulsed laser microbeam irradiation. Lysis to be mediated by laser-induced plasma formation was demonstrated by images obtained at time delays of 0.5 ns to 50 μs. The quantitative characterization of the pressure wave and cavitation bubble dynamics were enabled by image analysis. The results show that the zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansions as the primary agent of cell injury.",

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AB - The lysis of confluent PtK2 cell cultures was investigated by pulsed laser microbeam irradiation. Lysis to be mediated by laser-induced plasma formation was demonstrated by images obtained at time delays of 0.5 ns to 50 μs. The quantitative characterization of the pressure wave and cavitation bubble dynamics were enabled by image analysis. The results show that the zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansions as the primary agent of cell injury.

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Investigation of laser-induced cell lysis using time-resolved imaging

Abstract

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