TY - JOUR
T1 - Intraoperative differentiation of pancreatic neoplastic lesions using optical coherence tomography (OCT)
AU - Kist, Markus
AU - Strenge, Paul
AU - Keck, Tobias
AU - Weber, Andreas
AU - Bronsert, Peter
AU - Abdalla, Thaer S. A.
AU - Wellner, Ulrich Friedrich
AU - Thomaschewski, Michael
N1 - Publisher Copyright:
© The Author(s) 2025.
PY - 2025/7/18
Y1 - 2025/7/18
N2 - Purpose: The diagnostic methods for accurately differentiating the dignity of pancreatic neoplasms are limited. Worrisome features on MRI and endosonography guide the way to resection or conservative treatment with a relevant rate of failure. Intraoperative minimal invasive optical coherence tomography could be a solution for this challenge. The aim of this study is to investigate whether optical coherence tomography is suitable for differentiating of pancreatic neoplastic lesions. Methods: In this exploratory study, four patient’s specimens of pancreatic resections (white adipose tissue, intraductal papillary mucinous neoplasm (IPMN), pancreatic ductal adenocarcinoma (PDAC) based on IPMN and neuroendocrine pancreatic carcinoma) were prospectively examined ex vivo immediately after resection in the operating room using an optical coherence tomography system (Callisto 930nm, Thorlabs GmbH). In detail, the study investigated whether and in what way endocrine tumors, adenocarcinomas, premalignant and benign cysts differ morphologically in optical coherence tomography imaging compared to healthy pancreatic tissue. The final histopathological findings of the pancreatic specimens served as a reference and were correlated. Results: The samples examined ranged from typical fatty tissue, intraductal papillary mucinous neoplasm (IPMN), a moderate differentiated (G2) pancreatic ductal adenocarcinoma (PDAC) based on an intraductal papillary mucinous neoplasm (IPMN) and a neuroendocrine pancreatic carcinoma. Optical coherence tomography was feasible to replicate key histological characteristics and tissue architecture in correlation to conventional Hematoxylin-eosin histology. Conclusion: Optical coherence tomography imaging has the potential to differentiate between benign, pre-malignant and malignant pancreatic pathologies by morphology and should be examined in larger collectives.
AB - Purpose: The diagnostic methods for accurately differentiating the dignity of pancreatic neoplasms are limited. Worrisome features on MRI and endosonography guide the way to resection or conservative treatment with a relevant rate of failure. Intraoperative minimal invasive optical coherence tomography could be a solution for this challenge. The aim of this study is to investigate whether optical coherence tomography is suitable for differentiating of pancreatic neoplastic lesions. Methods: In this exploratory study, four patient’s specimens of pancreatic resections (white adipose tissue, intraductal papillary mucinous neoplasm (IPMN), pancreatic ductal adenocarcinoma (PDAC) based on IPMN and neuroendocrine pancreatic carcinoma) were prospectively examined ex vivo immediately after resection in the operating room using an optical coherence tomography system (Callisto 930nm, Thorlabs GmbH). In detail, the study investigated whether and in what way endocrine tumors, adenocarcinomas, premalignant and benign cysts differ morphologically in optical coherence tomography imaging compared to healthy pancreatic tissue. The final histopathological findings of the pancreatic specimens served as a reference and were correlated. Results: The samples examined ranged from typical fatty tissue, intraductal papillary mucinous neoplasm (IPMN), a moderate differentiated (G2) pancreatic ductal adenocarcinoma (PDAC) based on an intraductal papillary mucinous neoplasm (IPMN) and a neuroendocrine pancreatic carcinoma. Optical coherence tomography was feasible to replicate key histological characteristics and tissue architecture in correlation to conventional Hematoxylin-eosin histology. Conclusion: Optical coherence tomography imaging has the potential to differentiate between benign, pre-malignant and malignant pancreatic pathologies by morphology and should be examined in larger collectives.
UR - https://www.scopus.com/pages/publications/105011100694
UR - https://www.mendeley.com/catalogue/5761da99-8fff-36bd-9c92-7a780dfe2f7e/
U2 - 10.1007/s00423-025-03810-9
DO - 10.1007/s00423-025-03810-9
M3 - Journal articles
C2 - 40679633
SN - 1435-2451
VL - 410
SP - 227
JO - Langenbeck's Archives of Surgery
JF - Langenbeck's Archives of Surgery
IS - 1
M1 - 227
ER -
SDG 3 – Gesundheit und Wohlergehen