Interferon-α stimulation of liver cells enhances hepatitis delta virus RNA editing in early infection

Dirk Hartwig, Lutz Schoeneich, Jobst Greeve, Claudia Schütte, Isabel Dorn, Holger Kirchner, Holger Hennig

22 Zitate (Scopus)

Abstract

RNA editing controls the formation of hepatitis-delta-antigen-S and -L and therefore plays a central role in the hepatitis-delta-virus (HDV) life-cycle. Editing is catalyzed by the enzyme Adenosine-deaminase-acting-on-RNA1 (ADAR1) of which two different forms, ADAR1-L and ADAR1-S, exist. As ADAR1-L is induced by interferon (IFN)-α, we examined the influence of IFN-α-stimulation of host cells on HDV-RNA editing. Editing was studied in Huh-7-cells transfected with HDV-RNA on days 7, 14, 21 and 28 after transfection. ADAR1-L mRNA was measured by RT-PCR. IFN-α-treatment led to a 5-fold higher expression of ADAR1-L and to an increase in editing from 14±2% (SD) in unstimulated controls to 27±4% (SD) on day 7 after transfection. Editing further increases over time to the same maximum level of 35% in IFN-α-treated as well as untreated cells. By IFN-α-stimulation both ADAR1-L expression and editing are increased in Huh-7-cells at day 7, and the maximum level of edited antigenomes is reached earlier with IFN-α-treatment as compared to untreated cells. Thus, ADAR1-L appears to be able to increase editing, but the HDV genome apparently has an intrinsic negative feed-back regulation mechanism that limits editing to roughly a third of the genomes.

OriginalspracheEnglisch
ZeitschriftJournal of Hepatology
Jahrgang41
Ausgabenummer4
Seiten (von - bis)667-672
Seitenumfang6
ISSN0168-8278
DOIs
PublikationsstatusVeröffentlicht - 10.2004

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