Inducible and combinatorial gene manipulation in mouse brain

Godwin K. Dogbevia; Ricardo Marticorena-Alvarez; Melanie Bausen; Rolf Sprengel; Mazahir T. Hasan

doi:10.3389/fncel.2015.00142

Inducible and combinatorial gene manipulation in mouse brain

Godwin K. Dogbevia, Ricardo Marticorena-Alvarez, Melanie Bausen, Rolf Sprengel, Mazahir T. Hasan^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Experimentelle und Klinische Pharmakologie und Toxikologie

14 Zitate (Scopus)

Abstract

We have deployed recombinant adeno-associated viruses equipped with tetracyclinecontrolled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemicalgenetic approach will help to investigate ‘when’, ‘where’, and ‘how’ gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.

Originalsprache	Englisch
Zeitschrift	Frontiers in Cellular Neuroscience
Jahrgang	9
Ausgabenummer	APR
ISSN	1662-5102
DOIs	https://doi.org/10.3389/fncel.2015.00142
Publikationsstatus	Veröffentlicht - 21.04.2015

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)

Dokumente

10.3389/fncel.2015.00142

Weitere Links

Link to publication in Scopus

Zitieren

@article{f2e01379de3349c1aa6f6551c0854791,

title = "Inducible and combinatorial gene manipulation in mouse brain",

abstract = "We have deployed recombinant adeno-associated viruses equipped with tetracyclinecontrolled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemicalgenetic approach will help to investigate {\textquoteleft}when{\textquoteright}, {\textquoteleft}where{\textquoteright}, and {\textquoteleft}how{\textquoteright} gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.",

author = "Dogbevia, \{Godwin K.\} and Ricardo Marticorena-Alvarez and Melanie Bausen and Rolf Sprengel and Hasan, \{Mazahir T.\}",

year = "2015",

month = apr,

day = "21",

doi = "10.3389/fncel.2015.00142",

language = "English",

volume = "9",

journal = "Frontiers in Cellular Neuroscience",

issn = "1662-5102",

publisher = "Frontiers Media SA",

number = "APR",

}

TY - JOUR

T1 - Inducible and combinatorial gene manipulation in mouse brain

AU - Dogbevia, Godwin K.

AU - Marticorena-Alvarez, Ricardo

AU - Bausen, Melanie

AU - Sprengel, Rolf

AU - Hasan, Mazahir T.

PY - 2015/4/21

Y1 - 2015/4/21

N2 - We have deployed recombinant adeno-associated viruses equipped with tetracyclinecontrolled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemicalgenetic approach will help to investigate ‘when’, ‘where’, and ‘how’ gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.

AB - We have deployed recombinant adeno-associated viruses equipped with tetracyclinecontrolled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemicalgenetic approach will help to investigate ‘when’, ‘where’, and ‘how’ gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.

UR - https://www.scopus.com/pages/publications/84929587359

U2 - 10.3389/fncel.2015.00142

DO - 10.3389/fncel.2015.00142

M3 - Journal articles

AN - SCOPUS:84929587359

SN - 1662-5102

VL - 9

JO - Frontiers in Cellular Neuroscience

JF - Frontiers in Cellular Neuroscience

IS - APR

ER -

Inducible and combinatorial gene manipulation in mouse brain

Abstract

UN SDGs

Strategische Forschungsbereiche und Zentren

Dokumente

Weitere Links

Fingerprint

Zitieren