Immunoglobulin M pemphigoid

Katharina Boch; Christoph M. Hammers; Stephanie Goletz; Mayumi Kamaguchi; Ralf J. Ludwig; Stefan W. Schneider; Detlef Zillikens; Eva Hadaschik; Enno Schmidt

doi:10.1016/j.jaad.2021.01.017

Immunoglobulin M pemphigoid

Katharina Boch, Christoph M. Hammers, Stephanie Goletz, Mayumi Kamaguchi, Ralf J. Ludwig, Stefan W. Schneider, Detlef Zillikens, Eva Hadaschik, Enno Schmidt^*

^*Korrespondierende/r Autor/-in für diese Arbeit

26 Zitate (Scopus)

Abstract

Background: Pemphigoid diseases are a heterogeneous group of autoimmune blistering disorders characterized by predominant deposition of immunoglobulin G or immunoglobulin A autoantibodies against structural proteins of the dermoepidermal junction (DEJ). Sole linear immunoglobulin M (IgM) deposits at the DEJ in pemphigoid diseases have been observed; however, IgM-specific target antigens have not been identified. Objective: Characterization of patients with IgM pemphigoid. Methods: Skin biopsy specimens and sera from IgM-positive patients were assessed using histopathology, direct and indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, immunoblotting, cryosection assay, complement fixation test, and internalization assays. Results: Tissue-bound linear IgM deposits along the DEJ and circulating IgM autoantibodies against type XVII collagen (Col17) were detected. These circulating IgM autoantibodies showed no complement activating or blister inducing capacity, but the ability of Col17 internalization ex vivo. Limitations: Limited number of patients. Conclusion: This study provides further evidence for the role of IgM autoantibodies in pemphigoid disease and highlights Col17 as a target antigen in IgM pemphigoid.

Originalsprache	Englisch
Zeitschrift	Journal of the American Academy of Dermatology
Jahrgang	85
Ausgabenummer	6
Seiten (von - bis)	1486-1492
Seitenumfang	7
ISSN	0190-9622
DOIs	https://doi.org/10.1016/j.jaad.2021.01.017
Publikationsstatus	Veröffentlicht - 12.2021

Fördermittel

Funding sources: Supported by structural funding from the Deutsche Forschungsgemeinschaft through CRU 303 Pemphigoid Diseases and Excellence Cluster 2167/1 Precision Medicine in Chronic Inflammation (to Drs Ludwig, Zillikens, and Schmidt).We thank Ingeborg Atefi and Marina Kongsbak-Reim, L?beck, Germany, for excellent technical support, and Manuela Pigors for reviewing the manuscript. We are grateful to Christian Probst and Lars Komorowski, Euroimmun, L?beck, Germany, for providing recombinant Col17 proteins.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)
Zentren: Center for Research on Inflammation of the Skin (CRIS)

DFG-Fachsystematik

2.21-05 Immunologie
2.22-19 Dermatologie

Dokumente

10.1016/j.jaad.2021.01.017

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EXC 2167: Präzisionsmedizin für Chronische Entzündungserkrankungen (PMI)
Schreiber, S. (Sprecher*in), Baines, J. F. (Beteiligte*r Wissenschaftler*in), Bosch, T. C. G. (Beteiligte*r Wissenschaftler*in), Buyx, A. (Beteiligte*r Wissenschaftler*in), Erdmann, J. (Beteiligte*r Wissenschaftler*in), Franke, A. (Beteiligte*r Wissenschaftler*in), Huber, R. (Beteiligte*r Wissenschaftler*in), Klein, C. (Beteiligte*r Wissenschaftler*in), Köhl, J. (Beteiligte*r Wissenschaftler*in), König, I. R. (Beteiligte*r Wissenschaftler*in), Lange, C. (Beteiligte*r Wissenschaftler*in), Laudes, M. (Beteiligte*r Wissenschaftler*in), Lieb, W. (Beteiligte*r Wissenschaftler*in), Ludwig, R. (Beteiligte*r Wissenschaftler*in), Nebel, A. (Beteiligte*r Wissenschaftler*in), Niemann, S. (Sprecher*in), Rabe, K. F. (Beteiligte*r Wissenschaftler*in), Riemekasten, G. (Sprecher*in), Rose-John, S. (Beteiligte*r Wissenschaftler*in), Rosenstiel, P. C. (Beteiligte*r Wissenschaftler*in), Schulenburg, H. (Beteiligte*r Wissenschaftler*in), Schwarz, K. (Beteiligte*r Wissenschaftler*in), Traulsen, A. (Beteiligte*r Wissenschaftler*in), Weidinger, S. (Beteiligte*r Wissenschaftler*in) & Zillikens, D. (Beteiligte*r Wissenschaftler*in)
01.01.19 → 31.12.32
Projekt: DFG Verbundprojekte › Exzellenzcluster

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@article{2561e8e6be074608b4171f8f754daec4,

title = "Immunoglobulin M pemphigoid",

abstract = "Background: Pemphigoid diseases are a heterogeneous group of autoimmune blistering disorders characterized by predominant deposition of immunoglobulin G or immunoglobulin A autoantibodies against structural proteins of the dermoepidermal junction (DEJ). Sole linear immunoglobulin M (IgM) deposits at the DEJ in pemphigoid diseases have been observed; however, IgM-specific target antigens have not been identified. Objective: Characterization of patients with IgM pemphigoid. Methods: Skin biopsy specimens and sera from IgM-positive patients were assessed using histopathology, direct and indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, immunoblotting, cryosection assay, complement fixation test, and internalization assays. Results: Tissue-bound linear IgM deposits along the DEJ and circulating IgM autoantibodies against type XVII collagen (Col17) were detected. These circulating IgM autoantibodies showed no complement activating or blister inducing capacity, but the ability of Col17 internalization ex vivo. Limitations: Limited number of patients. Conclusion: This study provides further evidence for the role of IgM autoantibodies in pemphigoid disease and highlights Col17 as a target antigen in IgM pemphigoid.",

author = "Katharina Boch and Hammers, \{Christoph M.\} and Stephanie Goletz and Mayumi Kamaguchi and Ludwig, \{Ralf J.\} and Schneider, \{Stefan W.\} and Detlef Zillikens and Eva Hadaschik and Enno Schmidt",

note = "Publisher Copyright: {\textcopyright} 2021 American Academy of Dermatology, Inc. Copyright {\textcopyright} 2021 American Academy of Dermatology, Inc. Published by Elsevier Inc. All rights reserved.",

year = "2021",

month = dec,

doi = "10.1016/j.jaad.2021.01.017",

language = "English",

volume = "85",

pages = "1486--1492",

journal = "Journal of the American Academy of Dermatology",

issn = "0190-9622",

publisher = "Elsevier Inc.",

number = "6",

}

TY - JOUR

T1 - Immunoglobulin M pemphigoid

AU - Boch, Katharina

AU - Hammers, Christoph M.

AU - Goletz, Stephanie

AU - Kamaguchi, Mayumi

AU - Ludwig, Ralf J.

AU - Schneider, Stefan W.

AU - Zillikens, Detlef

AU - Hadaschik, Eva

AU - Schmidt, Enno

PY - 2021/12

Y1 - 2021/12

N2 - Background: Pemphigoid diseases are a heterogeneous group of autoimmune blistering disorders characterized by predominant deposition of immunoglobulin G or immunoglobulin A autoantibodies against structural proteins of the dermoepidermal junction (DEJ). Sole linear immunoglobulin M (IgM) deposits at the DEJ in pemphigoid diseases have been observed; however, IgM-specific target antigens have not been identified. Objective: Characterization of patients with IgM pemphigoid. Methods: Skin biopsy specimens and sera from IgM-positive patients were assessed using histopathology, direct and indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, immunoblotting, cryosection assay, complement fixation test, and internalization assays. Results: Tissue-bound linear IgM deposits along the DEJ and circulating IgM autoantibodies against type XVII collagen (Col17) were detected. These circulating IgM autoantibodies showed no complement activating or blister inducing capacity, but the ability of Col17 internalization ex vivo. Limitations: Limited number of patients. Conclusion: This study provides further evidence for the role of IgM autoantibodies in pemphigoid disease and highlights Col17 as a target antigen in IgM pemphigoid.

AB - Background: Pemphigoid diseases are a heterogeneous group of autoimmune blistering disorders characterized by predominant deposition of immunoglobulin G or immunoglobulin A autoantibodies against structural proteins of the dermoepidermal junction (DEJ). Sole linear immunoglobulin M (IgM) deposits at the DEJ in pemphigoid diseases have been observed; however, IgM-specific target antigens have not been identified. Objective: Characterization of patients with IgM pemphigoid. Methods: Skin biopsy specimens and sera from IgM-positive patients were assessed using histopathology, direct and indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, immunoblotting, cryosection assay, complement fixation test, and internalization assays. Results: Tissue-bound linear IgM deposits along the DEJ and circulating IgM autoantibodies against type XVII collagen (Col17) were detected. These circulating IgM autoantibodies showed no complement activating or blister inducing capacity, but the ability of Col17 internalization ex vivo. Limitations: Limited number of patients. Conclusion: This study provides further evidence for the role of IgM autoantibodies in pemphigoid disease and highlights Col17 as a target antigen in IgM pemphigoid.

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C2 - 33453342

AN - SCOPUS:85100805614

SN - 0190-9622

VL - 85

SP - 1486

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JO - Journal of the American Academy of Dermatology

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ER -

Immunoglobulin M pemphigoid

Abstract

Fördermittel

UN SDGs

Strategische Forschungsbereiche und Zentren

DFG-Fachsystematik

Dokumente

Weitere Links

Fingerprint

Projekte

EXC 2167: Präzisionsmedizin für Chronische Entzündungserkrankungen (PMI)

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