Immunoglobulin M pemphigoid

Katharina Boch; Christoph M. Hammers; Stephanie Goletz; Mayumi Kamaguchi; Ralf J. Ludwig; Stefan W. Schneider; Detlef Zillikens; Eva Hadaschik; Enno Schmidt

doi:10.1016/j.jaad.2021.01.017

Immunoglobulin M pemphigoid

Katharina Boch, Christoph M. Hammers, Stephanie Goletz, Mayumi Kamaguchi, Ralf J. Ludwig, Stefan W. Schneider, Detlef Zillikens, Eva Hadaschik, Enno Schmidt^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Abstract

Background: Pemphigoid diseases are a heterogeneous group of autoimmune blistering disorders characterized by predominant deposition of immunoglobulin G or immunoglobulin A autoantibodies against structural proteins of the dermoepidermal junction (DEJ). Sole linear immunoglobulin M (IgM) deposits at the DEJ in pemphigoid diseases have been observed; however, IgM-specific target antigens have not been identified. Objective: Characterization of patients with IgM pemphigoid. Methods: Skin biopsy specimens and sera from IgM-positive patients were assessed using histopathology, direct and indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, immunoblotting, cryosection assay, complement fixation test, and internalization assays. Results: Tissue-bound linear IgM deposits along the DEJ and circulating IgM autoantibodies against type XVII collagen (Col17) were detected. These circulating IgM autoantibodies showed no complement activating or blister inducing capacity, but the ability of Col17 internalization ex vivo. Limitations: Limited number of patients. Conclusion: This study provides further evidence for the role of IgM autoantibodies in pemphigoid disease and highlights Col17 as a target antigen in IgM pemphigoid.

Originalsprache	Englisch
Zeitschrift	Journal of the American Academy of Dermatology
Jahrgang	85
Ausgabenummer	6
Seiten (von - bis)	1486-1492
Seitenumfang	7
ISSN	0190-9622
DOIs	https://doi.org/10.1016/j.jaad.2021.01.017
Publikationsstatus	Veröffentlicht - 12.2021

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)
Zentren: Center for Research on Inflammation of the Skin (CRIS)

DFG-Fachsystematik

2.21-05 Immunologie
2.22-19 Dermatologie

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

Dokumente

10.1016/j.jaad.2021.01.017

Weitere Links

1 Abgeschlossen
1 Laufend

EXC 2167: Präzisionsmedizin für Chronische Entzündungserkrankungen (PMI)
Schreiber, S. (Sprecher*in, Koordinator*in), Baines, J. F. (Beteiligte*r Wissenschaftler*in), Bosch, T. C. G. (Beteiligte*r Wissenschaftler*in), Buyx, A. (Beteiligte*r Wissenschaftler*in), Erdmann, J. (Beteiligte*r Wissenschaftler*in), Franke, A. (Beteiligte*r Wissenschaftler*in), Huber, R. (Beteiligte*r Wissenschaftler*in), Klein, C. (Beteiligte*r Wissenschaftler*in), Köhl, J. (Beteiligte*r Wissenschaftler*in), König, I. R. (Beteiligte*r Wissenschaftler*in), Lange, C. (Beteiligte*r Wissenschaftler*in), Laudes, M. (Beteiligte*r Wissenschaftler*in), Lieb, W. (Beteiligte*r Wissenschaftler*in), Ludwig, R. (Beteiligte*r Wissenschaftler*in), Nebel, A. (Beteiligte*r Wissenschaftler*in), Niemann, S. (Beteiligte*r Wissenschaftler*in), Rabe, K. F. (Beteiligte*r Wissenschaftler*in), Riemekasten, G. (Beteiligte*r Wissenschaftler*in), Rose-John, S. (Beteiligte*r Wissenschaftler*in), Rosenstiel, P. C. (Beteiligte*r Wissenschaftler*in), Schulenburg, H. (Beteiligte*r Wissenschaftler*in), Schwarz, K. (Beteiligte*r Wissenschaftler*in), Traulsen, A. (Beteiligte*r Wissenschaftler*in), Weidinger, S. (Beteiligte*r Wissenschaftler*in) & Zillikens, D. (Beteiligte*r Wissenschaftler*in)
01.01.19 → …
Projekt: DFG-Projekte › DFG Exzellenzcluster
KFO 303: Pemphigoid Diseases - Molecular Pathways and their Therapeutic Potential
Sadik, C. (Sprecher*in, Koordinator*in), Zillikens, D. (Sprecher*in, Koordinator*in), Ibrahim, S. (Projektleiter*in (PI)), Baines, J. F. (Projektleiter*in (PI)), Schmidt, E. (Projektleiter*in (PI)), Köhl, J. (Projektleiter*in (PI)), Ehlers, M. (Projektleiter*in (PI)), Hirose, M. (Projektleiter*in (PI)), König, P. (Projektleiter*in (PI)), Ludwig, R. (Projektleiter*in (PI)), Manz, R. (Projektleiter*in (PI)), Schwaninger, M. (Projektleiter*in (PI)), Beek, N. (Projektleiter*in (PI)), Erdmann, J. (Projektleiter*in (PI)), König, I. R. (Projektleiter*in (PI)), Verschoor, A. (Projektleiter*in (PI)), Karsten, C. (Projektleiter*in (PI)), Bieber, K. (Projektleiter*in (PI)) & Busch, H. S. (Projektleiter*in (PI))
01.04.15 → 31.12.23
Projekt: DFG-Projekte › DFG-Verbundforschung: Forschergruppen/Klinische Forschergruppen

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@article{2561e8e6be074608b4171f8f754daec4,

title = "Immunoglobulin M pemphigoid",

abstract = "Background: Pemphigoid diseases are a heterogeneous group of autoimmune blistering disorders characterized by predominant deposition of immunoglobulin G or immunoglobulin A autoantibodies against structural proteins of the dermoepidermal junction (DEJ). Sole linear immunoglobulin M (IgM) deposits at the DEJ in pemphigoid diseases have been observed; however, IgM-specific target antigens have not been identified. Objective: Characterization of patients with IgM pemphigoid. Methods: Skin biopsy specimens and sera from IgM-positive patients were assessed using histopathology, direct and indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, immunoblotting, cryosection assay, complement fixation test, and internalization assays. Results: Tissue-bound linear IgM deposits along the DEJ and circulating IgM autoantibodies against type XVII collagen (Col17) were detected. These circulating IgM autoantibodies showed no complement activating or blister inducing capacity, but the ability of Col17 internalization ex vivo. Limitations: Limited number of patients. Conclusion: This study provides further evidence for the role of IgM autoantibodies in pemphigoid disease and highlights Col17 as a target antigen in IgM pemphigoid.",

author = "Katharina Boch and Hammers, {Christoph M.} and Stephanie Goletz and Mayumi Kamaguchi and Ludwig, {Ralf J.} and Schneider, {Stefan W.} and Detlef Zillikens and Eva Hadaschik and Enno Schmidt",

note = "Publisher Copyright: {\textcopyright} 2021 American Academy of Dermatology, Inc. Copyright {\textcopyright} 2021 American Academy of Dermatology, Inc. Published by Elsevier Inc. All rights reserved.",

year = "2021",

month = dec,

doi = "10.1016/j.jaad.2021.01.017",

language = "English",

volume = "85",

pages = "1486--1492",

journal = "Journal of the American Academy of Dermatology",

issn = "0190-9622",

number = "6",

}

TY - JOUR

T1 - Immunoglobulin M pemphigoid

AU - Boch, Katharina

AU - Hammers, Christoph M.

AU - Goletz, Stephanie

AU - Kamaguchi, Mayumi

AU - Ludwig, Ralf J.

AU - Schneider, Stefan W.

AU - Zillikens, Detlef

AU - Hadaschik, Eva

AU - Schmidt, Enno

PY - 2021/12

Y1 - 2021/12

N2 - Background: Pemphigoid diseases are a heterogeneous group of autoimmune blistering disorders characterized by predominant deposition of immunoglobulin G or immunoglobulin A autoantibodies against structural proteins of the dermoepidermal junction (DEJ). Sole linear immunoglobulin M (IgM) deposits at the DEJ in pemphigoid diseases have been observed; however, IgM-specific target antigens have not been identified. Objective: Characterization of patients with IgM pemphigoid. Methods: Skin biopsy specimens and sera from IgM-positive patients were assessed using histopathology, direct and indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, immunoblotting, cryosection assay, complement fixation test, and internalization assays. Results: Tissue-bound linear IgM deposits along the DEJ and circulating IgM autoantibodies against type XVII collagen (Col17) were detected. These circulating IgM autoantibodies showed no complement activating or blister inducing capacity, but the ability of Col17 internalization ex vivo. Limitations: Limited number of patients. Conclusion: This study provides further evidence for the role of IgM autoantibodies in pemphigoid disease and highlights Col17 as a target antigen in IgM pemphigoid.

AB - Background: Pemphigoid diseases are a heterogeneous group of autoimmune blistering disorders characterized by predominant deposition of immunoglobulin G or immunoglobulin A autoantibodies against structural proteins of the dermoepidermal junction (DEJ). Sole linear immunoglobulin M (IgM) deposits at the DEJ in pemphigoid diseases have been observed; however, IgM-specific target antigens have not been identified. Objective: Characterization of patients with IgM pemphigoid. Methods: Skin biopsy specimens and sera from IgM-positive patients were assessed using histopathology, direct and indirect immunofluorescence microscopy, enzyme-linked immunosorbent assays, immunoblotting, cryosection assay, complement fixation test, and internalization assays. Results: Tissue-bound linear IgM deposits along the DEJ and circulating IgM autoantibodies against type XVII collagen (Col17) were detected. These circulating IgM autoantibodies showed no complement activating or blister inducing capacity, but the ability of Col17 internalization ex vivo. Limitations: Limited number of patients. Conclusion: This study provides further evidence for the role of IgM autoantibodies in pemphigoid disease and highlights Col17 as a target antigen in IgM pemphigoid.

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Immunoglobulin M pemphigoid

Abstract

Strategische Forschungsbereiche und Zentren

DFG-Fachsystematik

UN SDGs

Dokumente

Weitere Links

Fingerprint

Projekte

EXC 2167: Präzisionsmedizin für Chronische Entzündungserkrankungen (PMI)

KFO 303: Pemphigoid Diseases - Molecular Pathways and their Therapeutic Potential

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