Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1

Karola Rittner; George Sczakiel

doi:10.1093/nar/19.7.1421

Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1

Karola Rittner, George Sczakiel^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Deutsches Krebsforschungszentrum

62 Zitate (Scopus)

Abstract

Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.

Originalsprache	Englisch
Zeitschrift	Nucleic Acids Research
Jahrgang	19
Ausgabenummer	7
Seiten (von - bis)	1421-1426
Seitenumfang	6
ISSN	0305-1048
DOIs	https://doi.org/10.1093/nar/19.7.1421
Publikationsstatus	Veröffentlicht - 11.04.1991

Fördermittel

We thank H. zur Hausen for continuous support, M. Pawlita for critical discussion and A. Kleinheinz for helpful advice for the microinjection experiments. We also thank V. Bosch for critically reading this manuscript. This work was supported in part by BMFT grant FKZ-II-083-89.

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title = "Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1",

abstract = "Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.",

author = "Karola Rittner and George Sczakiel",

note = "Funding Information: We thank H. zur Hausen for continuous support, M. Pawlita for critical discussion and A. Kleinheinz for helpful advice for the microinjection experiments. We also thank V. Bosch for critically reading this manuscript. This work was supported in part by BMFT grant FKZ-II-083-89.",

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doi = "10.1093/nar/19.7.1421",

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AU - Rittner, Karola

AU - Sczakiel, George

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N2 - Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.

AB - Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.

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DO - 10.1093/nar/19.7.1421

M3 - Journal articles

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AN - SCOPUS:0025872982

SN - 0305-1048

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JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 7

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Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1

Abstract

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