TY - JOUR
T1 - Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1
AU - Rittner, Karola
AU - Sczakiel, George
N1 - Funding Information:
We thank H. zur Hausen for continuous support, M. Pawlita for critical discussion and A. Kleinheinz for helpful advice for the microinjection experiments. We also thank V. Bosch for critically reading this manuscript. This work was supported in part by BMFT grant FKZ-II-083-89.
PY - 1991/4/11
Y1 - 1991/4/11
N2 - Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.
AB - Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.
UR - http://www.scopus.com/inward/record.url?scp=0025872982&partnerID=8YFLogxK
U2 - 10.1093/nar/19.7.1421
DO - 10.1093/nar/19.7.1421
M3 - Journal articles
C2 - 2027749
AN - SCOPUS:0025872982
SN - 0305-1048
VL - 19
SP - 1421
EP - 1426
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 7
ER -
