Hochauflösende 2D-Gelelektrophorese von Knochengewebsproben

Timo Gemoll; Tilman Laubert; Cornelius Grimme; Uwe J. Roblick; Jens K. Habermann

doi:10.1007/s12268-012-0224-6

Hochauflösende 2D-Gelelektrophorese von Knochengewebsproben

Timo Gemoll, Tilman Laubert, Cornelius Grimme, Uwe J. Roblick, Jens K. Habermann

Klinik für Chirurgie

Abstract

Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.

Originalsprache	Deutsch
Zeitschrift	BioSpektrum
Jahrgang	18
Ausgabenummer	5
Seiten (von - bis)	520-521
Seitenumfang	2
ISSN	0947-0867
DOIs	https://doi.org/10.1007/s12268-012-0224-6
Publikationsstatus	Veröffentlicht - 09.2012

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SDG 3 – Gesundheit und Wohlergehen
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Dokumente

10.1007/s12268-012-0224-6

http://www.mendeley.com/research/hochaufl%C3%B6sende-2dgelelektrophorese-von-knochengewebsproben

Zitieren

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title = "Hochaufl{\"o}sende 2D-Gelelektrophorese von Knochengewebsproben",

abstract = "Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.",

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AU - Gemoll, Timo

AU - Laubert, Tilman

AU - Grimme, Cornelius

AU - Roblick, Uwe J.

AU - Habermann, Jens K.

PY - 2012/9

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N2 - Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.

AB - Two-dimensional gel electrophoresis (2-DE) is commonly used to analyze and separate complex mixtures of intact proteins. It delivers a map of proteins which reflects changes in protein expression level, isoforms and post-translational modifications. In this protocol, we describe an optimized workflow with standardized sample preparation for the 2-DE to exploit all methodical and technical potentials.

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DO - 10.1007/s12268-012-0224-6

M3 - Zeitschriftenaufsätze

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