Abstract
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
| Originalsprache | Englisch |
|---|---|
| Zeitschrift | European Journal of Immunology |
| Jahrgang | 51 |
| Ausgabenummer | 12 |
| Seiten (von - bis) | 2708-3145 |
| Seitenumfang | 438 |
| ISSN | 0014-2980 |
| DOIs | |
| Publikationsstatus | Veröffentlicht - 12.2021 |
Fördermittel
Hyun-Dong Chang is supported by the Dr. Rolf M. Schwiete Foundation. Susanne Melzer and Attila Tarnok thank De Novo Software for providing FCS Express. Enrico Lugli is supported by a grant from the Associazione Italiana per la Ricerca sul Cancro (AIRC IG20676). Gabriele De Simone and Giovanni Galletti were supported by Fellowships from the Fondazione Italiana per la Ricerca sul Cancro-Associazione Italiana per la Ricerca sul Cancro (FIRC-AIRC). Jun Dong is supported by Deutsche Forschungsgemeinschft (DFG, German Research Foundation) Projektnummer 389687267 and Chinesisch-Deutsches Zentrum für Wissenschaftsförderung [Sino-German Center for Research Promotion (SGC)] grant C-0072. Nicola Gagliani, Samuel Huber and Franziska Muscate are supported by DFG fundings: SFB841,GA 2441/3-1, HU 1714/10-1. The tetramer APC-conjugated H-2K (d) HIV-1 gag197-205 AMQMLKETI used in TDS assay for mouse blood T cells was obtained through the NIH Tetramer Facility. Larissa Nogueira Almeida was supported by DFG research grant MA 2273/14-1. Supported by the following grants: AIRC 5X1000 2018 id. 21147 (Lorenzo Moretta); AIRC IG 2017 id. 19920 (Lorenzo Moretta); RC-2020 OPBG (Lorenzo Moretta); AIRC and European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 800924 (Linda Quatrini). Dirk Baumjohann was supported by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Emmy Noether Programme BA 5132/1-2 (252623821) and Germany's Excellence Strategy EXC2151 (390873048). The following formed the writing committees for the indicated sections: A. Graham Pockley, Gemma A. Foulds, Gabriele Multhoff (I.1Essential Controls and Reproducibility in Flow Cytometry); Susanne Melzer, Attila Tarnok (I.2 MIFlowCyt compliance and data display of flow cytometry data in immunological research); Florian Mair, Claudia Haftmann (I.3. Panel design in high-dimensional flow cytometry); Sara De Biasi, Lara Gibellini, Andrea Cossarizza (II. Rare cells – General rules); Samuele Notarbartolo, Daniela Latorre, Antonino Cassotta, Chiara Vasco, Federica Sallusto, Sergio Abrignani, Jens Geginat (III.1. Human conventional αβ CD4+ T cells); Regina Stark, Kylie Quinn, Jessica Borger (III.2 Murine Conventional αβ CD4+ T cells); Jasper J. P. van Beek, Gabriele De Simone, Giovanni Galletti, Enrico Lugli (III.3 Human conventional αβ CD8 T cells); Regina Stark, Kylie Quinn, Jessica Borger (III.4 Murine Conventional αβ CD8 T cells); Jun Dong (III.5 Human tissue resident memory T cells); Regina Stark, Klaas van Gisbergen (III.6 Murine tissue resident memory T cells); José Antonio Garrote, Aida Fiz, David Bernardo, Fernando Gabriel Chirdo, Eduardo Arranz (III.7 Human intestinal intraepithelial T cells); Immo Prinz, Joana Barros-Martins (III.8 Mouse intestinal intraepithelial T cells); Marcello Pinti (III.9 Immune senescence (aging) of human T cells); Luka Čičin-Šain, Katarzyna M. Sitnik, Kylie M. Quinn (III.10 Immune senescence (aging) in murine T cells); Dominic A Boardman, Eleni Christakou, Laura Cook, Michael Delacher, Markus Feuerer, Qing Huang, Sabine M Ivison, Megan K Levings, Katherine N. MacDonald, Timothy I.M. Tree, Kirsten A Ward-Hartstonge, Jennie H. M. Yang (III.11 Human FOXP3+ regulatory T cells); Michael Delacher, Juhao Yang, Jana Niemz, Lieke Sanderink, Markus Feuerer, Jochen Huehn (III.12 Murine Foxp3+ regulatory T cells); Chiara Vasco, Nadia Pulvirenti, Paola Gruarin, Jens Geginat (III.13 Human IL-10 producing regulatory T cells (Tr1 cells)); Franziska Muscate, Leonie Brockmann, Samuel Huber, Nicola Gagliani (III.14 Murine Tr1 cells); Anouk von Borstel, Martin S. Davey (III.15 Unconventional human T cells: gamma delta (γδ) T cells); Immo Prinz, Anneke Wilharm, Inga Sandrock (III.16 Unconventional murine T cells: γδ T cells); Christopher M. Harpur, Dale I. Godfrey, Garth Cameron (III.17 Unconventional human T cells: NKT cells); Andreas Krueger, Heike Kunze-Schumacher, Nikita A. Verheyden (III.18 Unconventional murine T cells: NKT cells); Garth Cameron, Nicholas A. Gherardin, Alexandra J. Corbett, Sidonia B. G. Eckle, James McCluskey, Dale I. Godfrey, Hui-Fern Koay (III.19 Unconventional human T cells: mucosal-Associated Invariant T (MAIT) cells); Andreas Krueger, Heike Kunze-Schumacher, Nikita A. Verheyden (III.20 Unconventional murine T cells: MAIT cells); Carina Saggau, Alexander Scheffold, Petra Bacher (IV.1 Antigen-specific T cell cytometry: Functional read-outs); Anastasia Gangaev, Steven Ketelaars, Pia Kvistborg (IV.2 Measuring antigen-specific CD8 T cell responses); Immanuel Andrä, Dirk Busch, Matthias Schiemann, Kilian Schober (IV.3 Antigen-specific T cell cytometry: MHC multimers); Alessio Mazzoni, Laura Maggi, Francesco Annunziato (IV.4 Identification of SARS-CoV-2 specific human CD4+ T cells by 8-color flow cytometry); Dirk Baumjohann, Yinshui Chang, Johanna E. Huber (IV. 5 Adoptive T cell transfers as a read-out for Ag-specific immune responses in mice); Maria B. Rodrigo, Nina Kessler, Laura Knop, Natalio Garbi, Thomas Schüler (IV.6 Cytotoxicity); Alessio Mazzoni, Laura Maggi, Francesco Annunziato (IV.7 Measurement of signal transduction pathways in human T cells); Alessio Mazzoni, Laura Maggi, Lorenzo Cosmi (IV.8 Live cytokine-producing cell sorting with cytokine secretion assayTM); Laura Maggi, Alessio Mazzoni, Francesco Liotta (IV.9 Quantification of soluble cytokines with cytometric bead array); James Wing, Ilenia Cammarata, Vincenzo Barnaba, Shimon Sakaguchi (IV.10 Treg suppression assays); Ambra Natalini, Sonia Simonetti, Giovanna Peruzzi, Miguel Muñoz-Ruiz, Leticia Monin, Irma Pujol-Autonell, Hefin Rhys, Heather M. Long, Adrian C. Hayday, Francesca Di Rosa (IV.11 Measurement of T cell proliferation by the TDS assay); Annika Wiedemann, Andreia Lino, Thomas Dörner (V.1 Human B cells and their subsets); Annika Wiedemann, Andreia C. Lino, H. Kristyanto, Hans U. Scherer, Thomas Dörner (V.2 Human antigen-specific B cells); Willem van de Veen, Oliver Wirz, Mübeccel Akdis (V.3 Human immunoglobulin heavy chain isotypes); Oliver Wirz, Willem van de Veen, Mübeccel Akdis (V.4 Human regulatory B cells); Larissa Nogueira Almeida, Rudolf Armin Manz (V.5 Murine B cells and their subsets, including Bregs); Antonia Niedobitek, Axel R. Schulz, Marie Burns, Julia Schulze, Henrik E. Mei (V.6 Human antibody-secreting plasmablasts and plasma cells); Katharina Kriegsmann, Leo Hansmann, Calin Manta, Michael Hundemer (V.7 Human plasma cells in multiple myeloma); Sebastian Schulz, Katharina Pracht, Jens Wittner, Dirk Mielenz, Wolfgang Schuh, Hans-Martin Jäck (V.8 Murine antibody-secreting plasmablasts and plasma cells); Franziska Szelinski, Annika Wiedemann, Andreia C. Lino, Thomas Dörner (VI.1 Measurement of signal transduction pathways in human B cells); Simon Fillatreau, Rudolf Armin Manz, Guido Heine (VI.2 Live cytokine-producing human B cell sorting with secretion assay: the case of IL-10); Baerbel Keller, Marie Follo, Klaus Warnatz (VI.3 Antigen-receptor mediated Ca2+ mobilization in lymphocytes); Dirk Baumjohann, Yinshui Chang, Johanna E. Huber (VI.4 Adoptive B cell transfers as a read-out for Ag-specific immune responses in mice); Daniela Carolina Hernández, Christina Stehle, Oliver Hoelsken, Andreas Diefenbach, James Di Santo, Chiara Romagnani (VII.1 Human Innate Lymphoid Cells); Christina Stehle, Daniela Carolina Hernández, Oliver Hoelsken, Andreas Diefenbach, James Di Santo, Chiara Romagnani (VII.2 Murine Innate Lymphoid Cells); Linda Quatrini, Lorenzo Moretta, Genny del Zotto (VIII.1 Human NK cells); Linda Quatrini, Lorenzo Moretta, Genny del Zotto (VIII.2 Murine NK cells); Regine J. Dress, Amit A. Patel, Charles-Antoine Dutertre, Florent Ginhoux (IX.1 Human mononuclear phagocytes: Monocytes, macrophages, and dendritic cells); Regine J. Dress, Amit A. Patel, Charles-Antoine Dutertre, Florent Ginhoux (IX.2 Murine mononuclear phagocytes: Monocytes, macrophages, and dendritic cells); Regine J. Dress, Amit A. Patel, Charles-Antoine Dutertre, Florent Ginhoux (X.1 Human mononuclear phagocytes: Monocytes, macrophages, and dendritic cells); Regine J. Dress, Amit A. Patel, Charles-Antoine Dutertre, Florent Ginhoux (X.2 Murine mononuclear phagocytes: Monocytes, macrophages, and dendritic cells); Janina Schoen, Christine Schauer, Jeeshan Singh, Jasmin Knopf, Luis Enrique Muñoz, Martin Herrmann (XI.1 Neutrophil, eosinophil, and basophil phenotypes); Janina Schoen, Christine Schauer, Jeeshan Singh, Jasmin Knopf, Luis Enrique Muñoz, Martin Herrmann (XI.2 Human bone marrow and cord blood neutrophils); Janina Schoen, Christine Schauer, Jeeshan Singh, Jasmin Knopf, Luis Enrique Muñoz, Martin Herrmann (XI.3 Murine bone marrow neutrophils); Frank A. Schildberg (XII.1 Human bone marrow stromal cells); Rebecca Cornelis, Richard Addo, Daniel Lenz, Mir-Farzin Mashreghi (XII.2 Murine bone marrow stromal cells); Peter K. Jani, Julia Tornack, Fritz Melchers, Gulce Itir Percin, Julia Fröbel, Claudia Waskow (XIII Hematopoietic stem cell phenotypes); Jenny F. Kuehne, Christine S. Falk (XIV Tumor cell phenotypes); Lennard Ostendorf, Chotima Böttcher, Sarah Starossom, Katja Kobow, Pascale Eede, Helena Radbruch (XV Brain and neural cell phenotypes); Laura J. Pallett, Mala K. Maini, Matteo Iannacone (XVI.1 Liver cell phenotypes); Katrin Neumann, Francesco Andreata, Cristian G. Beccaria, Matteo Iannacone (XVI.2 Murine liver cells). Hyun‐Dong Chang is supported by the Dr. Rolf M. Schwiete Foundation. Susanne Melzer and Attila Tarnok thank De Novo Software for providing FCS Express. Enrico Lugli is supported by a grant from the Associazione Italiana per la Ricerca sul Cancro (AIRC IG20676). Gabriele De Simone and Giovanni Galletti were supported by Fellowships from the Fondazione Italiana per la Ricerca sul Cancro‐Associazione Italiana per la Ricerca sul Cancro (FIRC‐AIRC). Jun Dong is supported by Deutsche Forschungsgemeinschft (DFG, German Research Foundation) Projektnummer 389687267 and Chinesisch‐Deutsches Zentrum für Wissenschaftsförderung [Sino‐German Center for Research Promotion (SGC)] grant C‐0072. Nicola Gagliani, Samuel Huber and Franziska Muscate are supported by DFG fundings: SFB841,GA 2441/3‐1, HU 1714/10‐1. The tetramer APC‐conjugated H‐2K (d) HIV‐1 gag AMQMLKETI used in TDS assay for mouse blood T cells was obtained through the NIH Tetramer Facility. Larissa Nogueira Almeida was supported by DFG research grant MA 2273/14‐1. Supported by the following grants: AIRC 5X1000 2018 id. 21147 (Lorenzo Moretta); AIRC IG 2017 id. 19920 (Lorenzo Moretta); RC‐2020 OPBG (Lorenzo Moretta); AIRC and European Union's Horizon 2020 research and innovation program under the Marie Skłodowska‐Curie grant agreement No 800924 (Linda Quatrini). Dirk Baumjohann was supported by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Emmy Noether Programme BA 5132/1‐2 (252623821) and Germany's Excellence Strategy EXC2151 (390873048). 197‐205
| Träger | Trägernummer |
|---|---|
| Fondazione Italiana per la Ricerca sul Cancro | |
| Alexander Scheffold | |
| Rebecca Cornelis | |
| Carina Saggau | |
| H2020 Marie Skłodowska-Curie Actions | |
| Anouk von Borstel | |
| V.7 Human plasma cells in multiple myeloma | |
| Chiara Vasco | |
| James Wing, Ilenia Cammarata | |
| Jenny F. Kuehne, Christine S. Falk | |
| Daniela Carolina Hernández | |
| Ambra Natalini | |
| Larissa Nogueira Almeida | |
| Regina Stark | |
| Rudolf Armin Manz | |
| Sonia Simonetti | |
| Petra Bacher | |
| Fondazione Italiana per la Ricerca sul Cancro-Associazione Italiana per la Ricerca sul Cancro | |
| Antonia Niedobitek | |
| Horizon 2020 | |
| Fondazione Italiana per la Ricerca sul Cancro-Associazione Italiana per la Ricerca sul Cancro | |
| Associazione Italiana per la Ricerca sul Cancro | IG20676 |
| Shenzhen Gas Corporation | GA 2441/3-1 |
| National Institutes of Health | 19920, 21147, 5X1000 2018, MA 2273/14-1 |
| Chinesisch-Deutsche Zentrum für Wissenschaftsförderung | C‐0072, SFB841, MA 2273/14‐1, HU 1714/10‐1 |
| Nicht hinzugefügt | MR/V02423X/1 |
| Deutsche Forschungsgemeinschaft | 390873048, 197‐205, 389687267, EXC2151, BA 5132/1‐2, 252623821 |
| Horizon 2020 Framework Programme | 800924 |
UN SDGs
Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung
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SDG 3 – Gesundheit und Wohlergehen
Strategische Forschungsbereiche und Zentren
- Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)
DFG-Fachsystematik
- 2.21-05 Immunologie
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