Genome-wide profiling of transcription factor activity in primary liver cancer using single-cell ATAC sequencing

Amanda J. Craig, Maruhen A.Datsch Silveira, Lichun Ma, Mahler Revsine, Limin Wang, Sophia Heinrich, Zachary Rae, Allison Ruchinskas, Kimia Dadkhah, Whitney Do, Shay Behrens, Farid R. Mehrabadi, Dana A. Dominguez, Marshonna Forgues, Anuradha Budhu, Jittiporn Chaisaingmongkol, Jonathan M. Hernandez, Jeremy L. Davis, Bao Tran, Jens U. MarquardtMathuros Ruchirawat, Michael Kelly, Tim F. Greten, Xin W. Wang*

*Korrespondierende/r Autor/-in für diese Arbeit

Abstract

Summary Primary liver cancer (PLC) consists of two main histological subtypes; hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA). The role of transcription factors (TFs) in malignant hepatobiliary lineage commitment between HCC and iCCA remains underexplored. Here, we present genome-wide profiling of transcription regulatory elements of 16 PLC patients using single-cell assay for transposase accessible chromatin sequencing. Single-cell open chromatin profiles reflect the compositional diversity of liver cancer, identifying both malignant and microenvironment component cells. TF motif enrichment levels of 31 TFs strongly discriminate HCC from iCCA tumors. These TFs are members of the nuclear/retinoid receptor, POU, or ETS motif families. POU factors are associated with prognostic features in iCCA. Overall, nuclear receptors, ETS and POU TF motif families delineate transcription regulation between HCC and iCCA tumors, which may be relevant to development and selection of PLC subtype-specific therapeutics.
OriginalspracheEnglisch
Aufsatznummer113446
ZeitschriftCell Reports
Jahrgang42
Ausgabenummer11
Seiten (von - bis)113446
ISSN2211-1247
DOIs
PublikationsstatusVeröffentlicht - 28.11.2023

Strategische Forschungsbereiche und Zentren

  • Profilbereich: Lübeck Integrated Oncology Network (LION)
  • Zentren: Universitäres Cancer Center Schleswig-Holstein (UCCSH)

DFG-Fachsystematik

  • 205-14 Hämatologie, Onkologie

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