Generation of human and murine dendritic cells

Dendritic cells (DC) are professional antigen-presenting cells (APC) that serve as sentinels for the induction and regulation of immune responses. After contact with antigenic material in the context of danger signals, they mature and upregulate major histocompatibility complexes and costimulatory molecules required for effective interaction with T cells. The development of techniques to generate large numbers of homogeneous DC populations in vitro from human progenitors allowed their use for research purposes as well as for clinical applications. Apply the cell suspension on top of the depletion column and allowed it to enter for 5 min. Wash the column six times and collect the flow through, which contains the desired cells. Place bones in 70% alcohol for 1 min, wash them with PBS, and place them in a 2-cm petri dish. Then, cut off both ends of the bones with the scalpel with gently sawing movements. Washes must be performed with extreme care to avoid dislodging too many DC.