Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Beate Kroll; Monica Emde; Andreas Jeromin; Lothar Penner; Gerhard Rechkemmer; Titus Kretzschmar; Andreas Klos; Jörg Köhl; Wilfried Bautsch

doi:10.1016/0014-5793(91)81285-G

Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Beate Kroll, Monica Emde, Andreas Jeromin, Lothar Penner, Gerhard Rechkemmer, Titus Kretzschmar, Andreas Klos, Jörg Köhl, Wilfried Bautsch^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Systemische Entzündungsforschung

12 Zitate (Scopus)

Abstract

cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

Originalsprache	Englisch
Zeitschrift	FEBS Letters
Jahrgang	291
Ausgabenummer	2
Seiten (von - bis)	208-210
Seitenumfang	3
ISSN	0014-5793
DOIs	https://doi.org/10.1016/0014-5793(91)81285-G
Publikationsstatus	Veröffentlicht - 21.10.1991

Fördermittel

Ackrtorv/cdgememW ccorcl~ally thank 5 Bitter-Sucrmannfo r contl-nuous supporta nd many helpful dIscussIonsT his work was supported by grants of the DFG SonderforschungsbereIc2h4 4‘Chromsche Entzdndungen’a nd the Cystrc Flbrosls Foundation

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10.1016/0014-5793(91)81285-G

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title = "Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA",

abstract = "cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.",

author = "Beate Kroll and Monica Emde and Andreas Jeromin and Lothar Penner and Gerhard Rechkemmer and Titus Kretzschmar and Andreas Klos and J{\"o}rg K{\"o}hl and Wilfried Bautsch",

year = "1991",

month = oct,

day = "21",

doi = "10.1016/0014-5793(91)81285-G",

language = "English",

volume = "291",

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T1 - Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

AU - Kroll, Beate

AU - Emde, Monica

AU - Jeromin, Andreas

AU - Penner, Lothar

AU - Rechkemmer, Gerhard

AU - Kretzschmar, Titus

AU - Klos, Andreas

AU - Köhl, Jörg

AU - Bautsch, Wilfried

PY - 1991/10/21

Y1 - 1991/10/21

N2 - cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

AB - cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevis oocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopus oocytes.

UR - https://www.scopus.com/pages/publications/0026049699

U2 - 10.1016/0014-5793(91)81285-G

DO - 10.1016/0014-5793(91)81285-G

M3 - Journal articles

C2 - 1718782

AN - SCOPUS:0026049699

SN - 0014-5793

VL - 291

SP - 208

EP - 210

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Functional expression of a human C5a receptor clone in Xenopus oocytes requires additional RNA

Abstract

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