FcεRI induces the tryptophan degradation pathway involved in regulating T cell responses

Dagmar Von Bubnoff*, Heike Matz, Christine Frahnert, Marie Luise Rao, Daniel Hanau, Henri De la Salle, Thomas Bieber

*Korrespondierende/r Autor/-in für diese Arbeit
82 Zitate (Scopus)


FcεRI is suspected to play a pivotal role in the pathophysiology of atopic disorders such as atopic dermatitis. In search for genes differentially regulated by FcεRI on APCs, a differential cDNA bank of receptor-stimulated and unstimulated monocytes was established. By means of suppression subtractive hybridization, we identified kynurenine 3-monooxygenase and subsequently indoleamine 2,3-dioxygenase (IDO) to be overexpressed in FcεRI-activated monocytes. IDO is the rate-limiting enzyme in the catabolism of the essential amino acid tryptophan. We show that cross-linking of FcεRI on monocytes results in low tryptophan concentrations associated with impaired T cell stimulatory capacity. Importantly, T cell suppression could be prevented by the addition of tryptophan or inhibition of IDO. Moreover, stimulation of T cells by FcεRI-activated monocytes was increased compared with T cell stimulation by nonactivated monocytes if exogenous supply of tryptophan was available. We speculate that the expression of IDO by FcεRI+ APCs in vivo allows these cells to regulate T cell responses in atopic disorders by inhibiting or stimulating T cell proliferation, depending on the metabolic environment.

ZeitschriftJournal of Immunology
Seiten (von - bis)1810-1816
PublikationsstatusVeröffentlicht - 15.08.2002


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