Fatty acids of type 2 diabetic serum decrease the stemness properties of human adipose-derived mesenchymal stem cells

Parisa Fayyazpour, Effat Alizadeh, Vahid Hosseini, Ashkan Kalantary-Charvadeh, Mitra Niafar, Vahideh Sadra, Zahra Norouzi, Aysan Saebnazar, Amir Mehdizadeh*, Masoud Darabi

*Korrespondierende/r Autor/-in für diese Arbeit
3 Zitate (Scopus)

Abstract

In type 2 diabetes, dyslipidemia and increased serum free fatty acids (FFAs) exacerbate the development of the disease through a negative effect on insulin secretion. Adipose-derived mesenchymal stem cells (AdMSCs) play a key role in regenerative medicine, and these cells can potentially be applied as novel therapeutic resources in the treatment of diabetes. In this study, AdMSCs were treated with diabetic or nondiabetic serum FFAs isolated from women of menopausal age. Serum FFAs were analyzed using gas–liquid chromatography. The expression level of the stemness markers CD49e and CD90 and the Wnt signaling target genes Axin-2 and c-Myc were evaluated using real-time PCR. The proliferation rate and colony formation were also assessed using a BrdU assay and crystal violet staining, respectively. The level of glutathione was assessed using cell fluorescence staining. Compared to nondiabetic serum, diabetic serum contained a higher percentage of oleate (1.5-fold, p < 0.01). In comparison with nondiabetic FFAs, diabetic FFAs demonstrated decreasing effects on the expression of CD90 (−51%, p < 0.001) and c-Myc (−48%, p < 0.05), and proliferation rate (−35%, p < 0.001), colony formation capacity (−50%, p < 0.01), and GSH levels (−62%, p < 0.05). The negative effect of the FFAs of diabetic serum on the stemness characteristics may impair the regenerative capabilities of AdMSCs.

OriginalspracheEnglisch
ZeitschriftJournal of Cellular Biochemistry
Jahrgang123
Ausgabenummer7
Seiten (von - bis)1157-1170
Seitenumfang14
ISSN0730-2312
DOIs
PublikationsstatusVeröffentlicht - 07.2022

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