TY - JOUR
T1 - Exogenous arachidonate restores the dimethoate-induced inhibition of steroidogenesis in rat interstitial cells
AU - Astiz, Mariana
AU - De Catalfo, Graciela Hurtado
AU - De Alaniz, María J.T.
AU - Marra, Carlos Alberto
N1 - Funding Information:
Acknowledgments This study was supported by a grant from Consejo Nacional de Investigaciones Científicas y Técnicas (CONI-CET), Argentina. We would like to thank Mrs. Agustina Zardis de Cobeñas, Eva Illara de Bozzolo, and Norma Cristalli for their excellent technical assistance.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2012/6
Y1 - 2012/6
N2 - The present work studies the potential restorative effect of polyunsaturated fatty acids (PUFA, 5 μM/24 h) on the dimethoate (DMT)-induced inhibition of testosterone biosynthesis in Leydig cells isolated from rat testes. Various fatty acids (FA) from the n-6 (18:2, 20:3, 20:4, 22:4 and 22:5) and n-3 (18.3, 20:5, 22:5, 22:6) series were assayed in Leydig cells, alone (as delipidated BSA complexes) and in combination with DMT (1 ppm). The n-6 FA stimulated lipid peroxidation (LPO) and inhibited the activities of steroidogenic enzymes (3β- and 17β-hydroxysteroid dehydrogenases). The n-3 FA exerted an anti-oxidant effect, decreasing the production of thiobarbituricacid reactive substances (TBARS) and inhibiting phospholipase A2 activity. The biosynthesis of testosterone in DMT-treated cultures was completely normalized by ARA (20:4n-6) and partially restored by the addition of 20:3n-6, increasing ARA content inside the mitochondria. The other FA assayed failed to restore androgenesis. COX-2 protein and prostaglandin F2α and E2 production were stimulated by 20:3n-6, ARA, 18:3n-3 and 20:5 n-3. COX-2 protein decreased upon addition of 22:5n-3 and 22:6n-3. StAR protein was increased by ARA and partially increased by 20:3n-6, likely due to its metabolic conversion into ARA. Both FA increased the mitochondrial cholesterol pool available for testosterone biosynthesis. The rate of androgenesis is likely the result of various regulatory factors acting concomitantly on the physiology of Leydig cells.
AB - The present work studies the potential restorative effect of polyunsaturated fatty acids (PUFA, 5 μM/24 h) on the dimethoate (DMT)-induced inhibition of testosterone biosynthesis in Leydig cells isolated from rat testes. Various fatty acids (FA) from the n-6 (18:2, 20:3, 20:4, 22:4 and 22:5) and n-3 (18.3, 20:5, 22:5, 22:6) series were assayed in Leydig cells, alone (as delipidated BSA complexes) and in combination with DMT (1 ppm). The n-6 FA stimulated lipid peroxidation (LPO) and inhibited the activities of steroidogenic enzymes (3β- and 17β-hydroxysteroid dehydrogenases). The n-3 FA exerted an anti-oxidant effect, decreasing the production of thiobarbituricacid reactive substances (TBARS) and inhibiting phospholipase A2 activity. The biosynthesis of testosterone in DMT-treated cultures was completely normalized by ARA (20:4n-6) and partially restored by the addition of 20:3n-6, increasing ARA content inside the mitochondria. The other FA assayed failed to restore androgenesis. COX-2 protein and prostaglandin F2α and E2 production were stimulated by 20:3n-6, ARA, 18:3n-3 and 20:5 n-3. COX-2 protein decreased upon addition of 22:5n-3 and 22:6n-3. StAR protein was increased by ARA and partially increased by 20:3n-6, likely due to its metabolic conversion into ARA. Both FA increased the mitochondrial cholesterol pool available for testosterone biosynthesis. The rate of androgenesis is likely the result of various regulatory factors acting concomitantly on the physiology of Leydig cells.
UR - http://www.scopus.com/inward/record.url?scp=84863448329&partnerID=8YFLogxK
U2 - 10.1007/s11745-012-3669-y
DO - 10.1007/s11745-012-3669-y
M3 - Journal articles
C2 - 22476691
AN - SCOPUS:84863448329
SN - 0024-4201
VL - 47
SP - 557
EP - 569
JO - Lipids
JF - Lipids
IS - 6
ER -
