Evidence for a Rieske-type FeS center in the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Stefan Anemüller; Christian L. Schmidt; Günter Schäfer; Miguel Teixeira

doi:10.1016/0014-5793(93)81328-W

Evidence for a Rieske-type FeS center in the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Stefan Anemüller^*, Christian L. Schmidt, Günter Schäfer, Miguel Teixeira

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Biochemie

Instituto de Tecnologia Quimica e Biologica - Univesidade Nova de Lisboa

28 Zitate (Scopus)

Abstract

A high-potential iron-sulfur cluster with characteristics similar to a Rieske-type center was detected in the plasma membrane of Sulfolobus acidocaldarius by EPR spectroscopy. In the reduced form this center has g-values of g_z = 2.031, g_y = 1.890 and g_x = 1.725 (g_av = 1.88, rhombicity = 0.37) and its reduction potential at pH 7.4 was determined to be +325 ± 10 mV. The archaebacterial cluster exhibits some unique properties, in comparison to eubacterial and eukaryotic Rieske-type centers. First, the reduction potential is pH-dependent in the range from pH 6.7 to 8.2. Second, the typical inhibitor of Rieske FeS centers, DBMIB, had no effect on the g-values of this cluster. The center is reducible by both NADH and succinate in the presence of cyanide, an inhibitor of the terminal oxidases. The possible role of a Rieske-type center in an organism lacking any c-type cytochromes is discussed.

Originalsprache	Englisch
Zeitschrift	FEBS Letters
Jahrgang	318
Ausgabenummer	1
Seiten (von - bis)	61-64
Seitenumfang	4
ISSN	0014-5793
DOIs	https://doi.org/10.1016/0014-5793(93)81328-W
Publikationsstatus	Veröffentlicht - 22.02.1993

Fördermittel

Acknowledgements: This work was supportedb y JNICT, Portugal (GrantS TRDA/C/BI0/416/92t o M.T.) andg rantsf rom theD eutsche Forschungsgemeinscha(tfot S.A. and G.S.). We thank Prof. A.V. Xavierf or criticald iscussionas ndD r. E. Bill (Ltibeck)f or runnmgt he EPR spectras hownin Fig. 5.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

Dokumente

10.1016/0014-5793(93)81328-W

Weitere Links

Link to publication in Scopus

Zitieren

@article{8d17f983ff1e439ba13e83acb49671fd,

title = "Evidence for a Rieske-type FeS center in the thermoacidophilic archaebacterium Sulfolobus acidocaldarius",

abstract = "A high-potential iron-sulfur cluster with characteristics similar to a Rieske-type center was detected in the plasma membrane of Sulfolobus acidocaldarius by EPR spectroscopy. In the reduced form this center has g-values of gz = 2.031, gy = 1.890 and gx = 1.725 (gav = 1.88, rhombicity = 0.37) and its reduction potential at pH 7.4 was determined to be +325 ± 10 mV. The archaebacterial cluster exhibits some unique properties, in comparison to eubacterial and eukaryotic Rieske-type centers. First, the reduction potential is pH-dependent in the range from pH 6.7 to 8.2. Second, the typical inhibitor of Rieske FeS centers, DBMIB, had no effect on the g-values of this cluster. The center is reducible by both NADH and succinate in the presence of cyanide, an inhibitor of the terminal oxidases. The possible role of a Rieske-type center in an organism lacking any c-type cytochromes is discussed.",

author = "Stefan Anem{\"u}ller and Schmidt, \{Christian L.\} and G{\"u}nter Sch{\"a}fer and Miguel Teixeira",

note = "Funding Information: Acknowledgements: This work was supportedb y JNICT, Portugal (GrantS TRDA/C/BI0/416/92t o M.T.) andg rantsf rom theD eutsche Forschungsgemeinscha(tfot S.A. and G.S.). We thank Prof. A.V. Xavierf or criticald iscussionas ndD r. E. Bill (Ltibeck)f or runnmgt he EPR spectras hownin Fig. 5. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1993",

month = feb,

day = "22",

doi = "10.1016/0014-5793(93)81328-W",

language = "English",

volume = "318",

pages = "61--64",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

number = "1",

}

TY - JOUR

T1 - Evidence for a Rieske-type FeS center in the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

AU - Anemüller, Stefan

AU - Schmidt, Christian L.

AU - Schäfer, Günter

AU - Teixeira, Miguel

N1 - Funding Information: Acknowledgements: This work was supportedb y JNICT, Portugal (GrantS TRDA/C/BI0/416/92t o M.T.) andg rantsf rom theD eutsche Forschungsgemeinscha(tfot S.A. and G.S.). We thank Prof. A.V. Xavierf or criticald iscussionas ndD r. E. Bill (Ltibeck)f or runnmgt he EPR spectras hownin Fig. 5. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1993/2/22

Y1 - 1993/2/22

N2 - A high-potential iron-sulfur cluster with characteristics similar to a Rieske-type center was detected in the plasma membrane of Sulfolobus acidocaldarius by EPR spectroscopy. In the reduced form this center has g-values of gz = 2.031, gy = 1.890 and gx = 1.725 (gav = 1.88, rhombicity = 0.37) and its reduction potential at pH 7.4 was determined to be +325 ± 10 mV. The archaebacterial cluster exhibits some unique properties, in comparison to eubacterial and eukaryotic Rieske-type centers. First, the reduction potential is pH-dependent in the range from pH 6.7 to 8.2. Second, the typical inhibitor of Rieske FeS centers, DBMIB, had no effect on the g-values of this cluster. The center is reducible by both NADH and succinate in the presence of cyanide, an inhibitor of the terminal oxidases. The possible role of a Rieske-type center in an organism lacking any c-type cytochromes is discussed.

AB - A high-potential iron-sulfur cluster with characteristics similar to a Rieske-type center was detected in the plasma membrane of Sulfolobus acidocaldarius by EPR spectroscopy. In the reduced form this center has g-values of gz = 2.031, gy = 1.890 and gx = 1.725 (gav = 1.88, rhombicity = 0.37) and its reduction potential at pH 7.4 was determined to be +325 ± 10 mV. The archaebacterial cluster exhibits some unique properties, in comparison to eubacterial and eukaryotic Rieske-type centers. First, the reduction potential is pH-dependent in the range from pH 6.7 to 8.2. Second, the typical inhibitor of Rieske FeS centers, DBMIB, had no effect on the g-values of this cluster. The center is reducible by both NADH and succinate in the presence of cyanide, an inhibitor of the terminal oxidases. The possible role of a Rieske-type center in an organism lacking any c-type cytochromes is discussed.

UR - https://www.scopus.com/pages/publications/0027446594

U2 - 10.1016/0014-5793(93)81328-W

DO - 10.1016/0014-5793(93)81328-W

M3 - Journal articles

C2 - 8382170

AN - SCOPUS:0027446594

SN - 0014-5793

VL - 318

SP - 61

EP - 64

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Evidence for a Rieske-type FeS center in the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Abstract

Fördermittel

UN SDGs

Strategische Forschungsbereiche und Zentren

Dokumente

Weitere Links

Fingerprint

Zitieren