Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion

Helmut Brosterhus; Susanne Brings; Heike Leyendeckers; Rudolf A. Manz; Stefan Miltenyi; Andreas Radbruch; Mario Assenmacher; Jürgen Schmitz

doi:10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C

Enrichment and detection of live antigen-specific CD4⁺ and CD8⁺ T cells based on cytokine secretion

Helmut Brosterhus, Susanne Brings, Heike Leyendeckers, Rudolf A. Manz, Stefan Miltenyi, Andreas Radbruch, Mario Assenmacher, Jürgen Schmitz^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Systemische Entzündungsforschung

185 Zitate (Scopus)

Abstract

Following appropriate antigen-specific stimulation, CD4⁺ and CD8⁺ T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4⁺ and CD8⁺ T lymphocytes. The power of this technique is demonstrated here for HL4-A0201-restricted influenza matrix protein peptide 58-66-specific CD8⁺ cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.

Originalsprache	Englisch
Zeitschrift	European Journal of Immunology
Jahrgang	29
Ausgabenummer	12
Seiten (von - bis)	4053-4059
Seitenumfang	7
ISSN	0014-2980
DOIs	https://doi.org/10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C
Publikationsstatus	Veröffentlicht - 1999

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SDG 3 – Gesundheit und Wohlergehen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

Dokumente

10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C

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title = "Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion",

abstract = "Following appropriate antigen-specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HL4-A0201-restricted influenza matrix protein peptide 58-66-specific CD8+ cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.",

author = "Helmut Brosterhus and Susanne Brings and Heike Leyendeckers and Manz, \{Rudolf A.\} and Stefan Miltenyi and Andreas Radbruch and Mario Assenmacher and J{\"u}rgen Schmitz",

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T1 - Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion

AU - Brosterhus, Helmut

AU - Brings, Susanne

AU - Leyendeckers, Heike

AU - Manz, Rudolf A.

AU - Miltenyi, Stefan

AU - Radbruch, Andreas

AU - Assenmacher, Mario

AU - Schmitz, Jürgen

PY - 1999

Y1 - 1999

N2 - Following appropriate antigen-specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HL4-A0201-restricted influenza matrix protein peptide 58-66-specific CD8+ cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.

AB - Following appropriate antigen-specific stimulation, CD4+ and CD8+ T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4+ and CD8+ T lymphocytes. The power of this technique is demonstrated here for HL4-A0201-restricted influenza matrix protein peptide 58-66-specific CD8+ cytotoxic T lymphocytes, influenza A virus- and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.

UR - https://www.scopus.com/pages/publications/0032729496

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DO - 10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.0.CO;2-C

M3 - Journal articles

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AN - SCOPUS:0032729496

SN - 0014-2980

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JO - European Journal of Immunology

JF - European Journal of Immunology

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