Embryonic stem cell-derived chondrogenic differentiation in vitro: Activation by BMP-2 and BMP-4

Jan Kramer; Claudia Hegert; Kaomei Guan; Anna M. Wobus; Peter K. Müller; Jürgen Rohwedel

doi:10.1016/S0925-4773(99)00339-1

Embryonic stem cell-derived chondrogenic differentiation in vitro: Activation by BMP-2 and BMP-4

Jan Kramer, Claudia Hegert, Kaomei Guan, Anna M. Wobus, Peter K. Müller, Jürgen Rohwedel^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Virologie und Zellbiologie

In Vitro Differentiation Group

383 Zitate (Scopus)

Abstract

Differentiation of mouse embryonic stem (ES) cells via embryoid bodies was established as a suitable model to study development in vitro. Here, we show that differentiation of ES cells in vitro into chondrocytes can be modulated by members of the transforming growth factor-β family (TGF-β₁, BMP-2 and -4). ES cell differentiation into chondrocytes was characterized by the appearance of Alcian blue-stained areas and the expression of cartilage- associated genes and proteins. Different stages of cartilage differentiation could be distinguished according to the expression pattern of the transcription factor scleraxis, and the cartilage matrix protein collagen II. The number of Alcian-blue-stained areas decreased slightly after application of TGF-β₁, whereas BMP-2 or -4 induced chondrogenic differentiation. The inducing effect of BMP-2 was found to be dependent on the time of application, consistent with its role to recruit precursor cells to the chondrogenic fate. (C) 2000 Elsevier Science Ireland Ltd.

Originalsprache	Englisch
Zeitschrift	Mechanisms of Development
Jahrgang	92
Ausgabenummer	2
Seiten (von - bis)	193-205
Seitenumfang	13
ISSN	0925-4773
DOIs	https://doi.org/10.1016/S0925-4773(99)00339-1
Publikationsstatus	Veröffentlicht - 01.04.2000

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The skilful technical assistance of S. Vandersee and B. Lembrich is gratefully acknowledged. The authors would like to thank Genetics Institute (Cambridge, MA, USA.) for the gift of recombinant human BMP-2 and BMP-4 protein and M. Paulsson (University of Cologne, Dept. of Biochemistry, Germany) for the anti-COMP antibody. The II-II 6B3 monoclonal antibody developed by T.F. Linsenmayer was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA52242. The work was supported by the Deutsche Forschungsgemeinschaft (Ro 2108/1-1).

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Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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10.1016/S0925-4773(99)00339-1

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title = "Embryonic stem cell-derived chondrogenic differentiation in vitro: Activation by BMP-2 and BMP-4",

abstract = "Differentiation of mouse embryonic stem (ES) cells via embryoid bodies was established as a suitable model to study development in vitro. Here, we show that differentiation of ES cells in vitro into chondrocytes can be modulated by members of the transforming growth factor-β family (TGF-β1, BMP-2 and -4). ES cell differentiation into chondrocytes was characterized by the appearance of Alcian blue-stained areas and the expression of cartilage- associated genes and proteins. Different stages of cartilage differentiation could be distinguished according to the expression pattern of the transcription factor scleraxis, and the cartilage matrix protein collagen II. The number of Alcian-blue-stained areas decreased slightly after application of TGF-β1, whereas BMP-2 or -4 induced chondrogenic differentiation. The inducing effect of BMP-2 was found to be dependent on the time of application, consistent with its role to recruit precursor cells to the chondrogenic fate. (C) 2000 Elsevier Science Ireland Ltd.",

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AU - Kramer, Jan

AU - Hegert, Claudia

AU - Guan, Kaomei

AU - Wobus, Anna M.

AU - Müller, Peter K.

AU - Rohwedel, Jürgen

PY - 2000/4/1

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N2 - Differentiation of mouse embryonic stem (ES) cells via embryoid bodies was established as a suitable model to study development in vitro. Here, we show that differentiation of ES cells in vitro into chondrocytes can be modulated by members of the transforming growth factor-β family (TGF-β1, BMP-2 and -4). ES cell differentiation into chondrocytes was characterized by the appearance of Alcian blue-stained areas and the expression of cartilage- associated genes and proteins. Different stages of cartilage differentiation could be distinguished according to the expression pattern of the transcription factor scleraxis, and the cartilage matrix protein collagen II. The number of Alcian-blue-stained areas decreased slightly after application of TGF-β1, whereas BMP-2 or -4 induced chondrogenic differentiation. The inducing effect of BMP-2 was found to be dependent on the time of application, consistent with its role to recruit precursor cells to the chondrogenic fate. (C) 2000 Elsevier Science Ireland Ltd.

AB - Differentiation of mouse embryonic stem (ES) cells via embryoid bodies was established as a suitable model to study development in vitro. Here, we show that differentiation of ES cells in vitro into chondrocytes can be modulated by members of the transforming growth factor-β family (TGF-β1, BMP-2 and -4). ES cell differentiation into chondrocytes was characterized by the appearance of Alcian blue-stained areas and the expression of cartilage- associated genes and proteins. Different stages of cartilage differentiation could be distinguished according to the expression pattern of the transcription factor scleraxis, and the cartilage matrix protein collagen II. The number of Alcian-blue-stained areas decreased slightly after application of TGF-β1, whereas BMP-2 or -4 induced chondrogenic differentiation. The inducing effect of BMP-2 was found to be dependent on the time of application, consistent with its role to recruit precursor cells to the chondrogenic fate. (C) 2000 Elsevier Science Ireland Ltd.

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Embryonic stem cell-derived chondrogenic differentiation in vitro: Activation by BMP-2 and BMP-4

Abstract

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UN SDGs

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