Effect of pesticides on cell survival in liver and brain rat tissues

Mariana Astiz; María J.T.de Alaniz; Carlos Alberto Marra

doi:10.1016/j.ecoenv.2009.05.001

Effect of pesticides on cell survival in liver and brain rat tissues

Mariana Astiz, María J.T.de Alaniz, Carlos Alberto Marra^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Neurobiologie

Universität in La Plata

104 Zitate (Scopus)

Abstract

Pesticides are the main environmental factor associated with the etiology of human neurodegenerative disorders such as Parkinson's disease. Our laboratory has previously demonstrated that the treatment of rats with low doses of dimethoate, zineb or glyphosate alone or in combination induces oxidative stress (OS) in liver and brain. The aim of the present work was to investigate if the pesticide-induced OS was able to affect brain and liver cell survival. The treatment of Wistar rats with the pesticides (i.p. 1/250 LD50, three times a week for 5 weeks) caused loss of mitochondrial transmembrane potential and cardiolipin content, especially in substantia nigra (SN), with a concomitant increase of fatty acid peroxidation. The activation of calpain apoptotic cascade (instead of the caspase-dependent pathway) would be responsible for the DNA fragmentation pattern observed. Thus, these results may contribute to understand the effect(s) of chronic and simultaneous exposure to pesticides on cell survival.

Originalsprache	Englisch
Zeitschrift	Ecotoxicology and Environmental Safety
Jahrgang	72
Ausgabenummer	7
Seiten (von - bis)	2025-2032
Seitenumfang	8
ISSN	0147-6513
DOIs	https://doi.org/10.1016/j.ecoenv.2009.05.001
Publikationsstatus	Veröffentlicht - 10.2009

Fördermittel

This study was supported by a grant from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Universidad Nacional de La Plata (UNLP). We would like to thank Miss Norma Cristalli for her excellent technical assistance.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen
SDG 8 – Angemessene Arbeitsbedingungen und wirtschaftliches Wachstum
SDG 10 – Weniger Ungleichheiten

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)

Dokumente

10.1016/j.ecoenv.2009.05.001

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title = "Effect of pesticides on cell survival in liver and brain rat tissues",

abstract = "Pesticides are the main environmental factor associated with the etiology of human neurodegenerative disorders such as Parkinson's disease. Our laboratory has previously demonstrated that the treatment of rats with low doses of dimethoate, zineb or glyphosate alone or in combination induces oxidative stress (OS) in liver and brain. The aim of the present work was to investigate if the pesticide-induced OS was able to affect brain and liver cell survival. The treatment of Wistar rats with the pesticides (i.p. 1/250 LD50, three times a week for 5 weeks) caused loss of mitochondrial transmembrane potential and cardiolipin content, especially in substantia nigra (SN), with a concomitant increase of fatty acid peroxidation. The activation of calpain apoptotic cascade (instead of the caspase-dependent pathway) would be responsible for the DNA fragmentation pattern observed. Thus, these results may contribute to understand the effect(s) of chronic and simultaneous exposure to pesticides on cell survival.",

author = "Mariana Astiz and Alaniz, \{Mar{\'i}a J.T.de\} and Marra, \{Carlos Alberto\}",

note = "Funding Information: This study was supported by a grant from Consejo Nacional de Investigaciones Cient{\'i}ficas y T{\'e}cnicas (CONICET) and Universidad Nacional de La Plata (UNLP). We would like to thank Miss Norma Cristalli for her excellent technical assistance. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

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AU - Astiz, Mariana

AU - Alaniz, María J.T.de

AU - Marra, Carlos Alberto

N1 - Funding Information: This study was supported by a grant from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Universidad Nacional de La Plata (UNLP). We would like to thank Miss Norma Cristalli for her excellent technical assistance. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2009/10

Y1 - 2009/10

N2 - Pesticides are the main environmental factor associated with the etiology of human neurodegenerative disorders such as Parkinson's disease. Our laboratory has previously demonstrated that the treatment of rats with low doses of dimethoate, zineb or glyphosate alone or in combination induces oxidative stress (OS) in liver and brain. The aim of the present work was to investigate if the pesticide-induced OS was able to affect brain and liver cell survival. The treatment of Wistar rats with the pesticides (i.p. 1/250 LD50, three times a week for 5 weeks) caused loss of mitochondrial transmembrane potential and cardiolipin content, especially in substantia nigra (SN), with a concomitant increase of fatty acid peroxidation. The activation of calpain apoptotic cascade (instead of the caspase-dependent pathway) would be responsible for the DNA fragmentation pattern observed. Thus, these results may contribute to understand the effect(s) of chronic and simultaneous exposure to pesticides on cell survival.

AB - Pesticides are the main environmental factor associated with the etiology of human neurodegenerative disorders such as Parkinson's disease. Our laboratory has previously demonstrated that the treatment of rats with low doses of dimethoate, zineb or glyphosate alone or in combination induces oxidative stress (OS) in liver and brain. The aim of the present work was to investigate if the pesticide-induced OS was able to affect brain and liver cell survival. The treatment of Wistar rats with the pesticides (i.p. 1/250 LD50, three times a week for 5 weeks) caused loss of mitochondrial transmembrane potential and cardiolipin content, especially in substantia nigra (SN), with a concomitant increase of fatty acid peroxidation. The activation of calpain apoptotic cascade (instead of the caspase-dependent pathway) would be responsible for the DNA fragmentation pattern observed. Thus, these results may contribute to understand the effect(s) of chronic and simultaneous exposure to pesticides on cell survival.

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M3 - Journal articles

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SN - 0147-6513

VL - 72

SP - 2025

EP - 2032

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

IS - 7

ER -

Effect of pesticides on cell survival in liver and brain rat tissues

Abstract

Fördermittel

UN SDGs

Strategische Forschungsbereiche und Zentren

Dokumente

Weitere Links

Fingerprint

Zitieren