Effect of matrix elasticity on the maintenance of the chondrogenic phenotype

Elena Schuh, Jan Kramer, Jürgen Rohwedel, Holger Notbohm, Ralph Müller, Thomas Gutsmann, Nicole Rotter*

*Korrespondierende/r Autor/-in für diese Arbeit
50 Zitate (Scopus)

Abstract

The aim of this study was to examine the influence of matrix elasticity on the maintenance of the chondrogenic phenotype of chondrocytes cultured in monolayer. We used a two-dimensional culturing system in which polyacrylamide gels with different concentrations of bis-acrylamide were coated with collagen type I. Matrices with a Young's modulus of 4, 10, 40, and 100 kPa were produced, as determined by atomic force microscopy. Porcine chondrocytes were cultivated on these matrices at a low density for 7 days. The proliferation of cells was analyzed by 5-Bromo-2′-deoxy-uridine incorporation. Maintenance of the chondrogenic phenotype was analyzed by measuring collagen type I, type II, and aggrecan gene expression, immunofluorescence staining for collagen type II, and phalloidin staining for actin filaments. Cellular proliferation and actin organization were decreased on matrices of 4 kPa compared with stiffer substrates. The differentiated phenotype of the chondrocytes grown on matrices of 4 kPa was stabilized, indicated by higher collagen type II and aggrecan, and lower collagen type I expression. These findings indicate that chondrocytes sense the elasticity of the matrix and might be used for the design of scaffolds with mechanical properties specifically tailored to support the chondrogenic phenotype in tissue engineering applications.

OriginalspracheEnglisch
ZeitschriftTissue Engineering - Part A
Jahrgang16
Ausgabenummer4
Seiten (von - bis)1281-1290
Seitenumfang10
ISSN1937-3341
DOIs
PublikationsstatusVeröffentlicht - 01.04.2010

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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