Domain Structure of the Selenocysteine-specific Translation Factor SelB in Prokaryotes

August Böck*, Rolf Hilgenfeld, Peter Tormay, Reinhard Wilting, Matthias Kromayer

*Korrespondierende/r Autor/-in für diese Arbeit
6 Zitate (Scopus)

Abstract

Translation factor SelB is the key component for the specific decoding of UGA codons with selenocysteine at the ribosome. SelB binds selenocysteyl-tRNASec, guanine nucleotides and a secondary structure of the selenoprotein mRNA following the UGA at the 3′ side. A comparison of the amino acid sequences of SelB species from E. coli, Desulfomicrobium baculatum, Clostridium thermoaceticum and Haemophilus influenzae showed that the proteins consist of at least four structural domains from which the N-terminal three are well conserved and share homology with elongation factor Tu whereas the C-terminal one is more variable and displays no similarity to any protein known. With the aid of the coordinates of EF-Tu the N-terminal part has been modelled into a 3D structure which exhibits intriguing features concerning its interaction with guanine nucleotides and other components of the translational apparatus. Cloning and expression of fragments of SelB and biochemical analysis of the purified truncated proteins showed that the C-terrninal 19 kDa protein fragment is able to specifically bind to the selenoprotein mRNA. SelB, thus, is a translation factor functionally homologous to EF-Tu hooked up to the mRNA with its C-terminal end. The formation by SelB of a quaternary complex in vivo has been proven by overexpression of truncated genes of SelB and by demonstration that fragments comprising the mRNA or the tRNA binding domain inhibit selenocysteine insertion.

OriginalspracheEnglisch
ZeitschriftBiomedical and Environmental Sciences
Jahrgang10
Ausgabenummer2-3
Seiten (von - bis)125-128
Seitenumfang4
ISSN0895-3988
PublikationsstatusVeröffentlicht - 09.1997

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

Fingerprint

Untersuchen Sie die Forschungsthemen von „Domain Structure of the Selenocysteine-specific Translation Factor SelB in Prokaryotes“. Zusammen bilden sie einen einzigartigen Fingerprint.

Zitieren