Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment

Martin Deichmann*, Georg Sczakiel, Rainer Haas

*Korrespondierende/r Autor/-in für diese Arbeit
    1 Zitat (Scopus)


    To inactivate cell-associated and extracellular HIV-1 while preserving cellular surface antigens, a procedure was used based on PUVA treatment, i.e. addition of psoralen to cell suspensions followed by irradiation with UVA light. T-lymphoid MT-4 cells were infected with HIV-1 strain NL4-3,4'-aminomethyl-4,5',8-trimethylpsoralen was added, and the cell suspension was irradiated with 20 mW/cm2 UVA light for 3, 4 and 5 min. To evaluate virus inactivation, cells and supernatants were diluted serially and cocultured with uninfected MT-4 cells. Infectious HIV-1 was detected by cytopathic effects, immunofluorescence and p24 antigen ELISA. UVA irradiation at 3.6 J/cm2 (3 min 20 mW/cm2) reduced the amounts of both cell-associated and extracellular infectious HIV-1 by more than five orders of magnitude. Even at more stringent conditions of PUVA treatment (10 min 20 mW/cm2 UVA irradiation), conformational cellular surface epitopes remained detectable by flow cytometry.

    ZeitschriftJournal of Virological Methods
    Seiten (von - bis)89-95
    PublikationsstatusVeröffentlicht - 10.1997


    Untersuchen Sie die Forschungsthemen von „Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment“. Zusammen bilden sie einen einzigartigen Fingerprint.