Differential response between histological and biochemical biomarkers in the apple snail Pomacea canaliculata (Gasteropoda: Amullariidae) exposed to cypermethrin

F. Arrighetti; E. Ambrosio; M. Astiz; A. Rodrigues Capítulo; S. Lavarías

doi:10.1016/j.aquatox.2017.11.014

Differential response between histological and biochemical biomarkers in the apple snail Pomacea canaliculata (Gasteropoda: Amullariidae) exposed to cypermethrin

F. Arrighetti, E. Ambrosio, M. Astiz, A. Rodrigues Capítulo, S. Lavarías^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Neurobiologie

Universität in La Plata

46 Zitate (Scopus)

Abstract

To develop effective programs to monitor water quality is necessary to identify sensitive biomarkers in indicator species. The aim of this study was to evaluate different biomarkers in the apple snail Pomacea canaliculata exposed to the insecticide Cypermethrin (CYP). Adult male and female snails were exposed to sublethal CYP concentrations (10, 25 and 100 μg l⁻¹) for 1, 4, 7 and 14 days. The recovery of the exposed snails was also studied by a post-exposure assay. The activities of the enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST), the levels of lipid peroxidation (LPO) and protein oxidation (PC) in digestive gland and gills were studied as biomarkers of exposure. Histopathological changes in target tissues were also evaluated. In digestive gland, CYP caused a significant increase in SOD, CAT and GST activities compared to control (p < 0.05) as well as in LPO and PC levels (p < 0.05). However, such biochemical effects were neither concentration nor time dependent. Histopatological changes were observed in the exposed groups, such as an increase in the number of basophilic cells, hemocytic infiltration and epithelia atrophy. Additionally, a positive correlation between the surface occupied by pigmented corpuscles and CYP concentrations was observed at all exposure periods. Gills showed greater sensitivity to oxidative damage than digestive gland. CYP caused an acute toxic effect in LPO levels in this respiratory organ. The gill filament of exposed snails, exhibited a reduction or loss of cilia, vacuolization of the columnar cells and an increase in haemocyte content irrespective of the concentration. High concentrations of CYP caused disruptions in the columnar muscle fibers. In general, snails did not show an improvement in their basal state during post-exposure treatment. Apparently, males and females do not have differential sensitivity to the pesticide. The results of this study suggest that histopathological changes are the most sensitive time- and dose-dependent biomarkers of toxicity induced by CYP in P. canaliculata.

Originalsprache	Englisch
Zeitschrift	Aquatic Toxicology
Jahrgang	194
Seiten (von - bis)	140-151
Seitenumfang	12
ISSN	0166-445X
DOIs	https://doi.org/10.1016/j.aquatox.2017.11.014
Publikationsstatus	Veröffentlicht - 01.2018

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This work was partially supported by grants from Agencia Nacional de Promoción Científica y Técnica, Argentina ( PICT 2014-0810 and PICT 2015-0669 ) to Dr. A. Rodrigues Capítulo and Dr. F. Arrighetti respectively, and grant from CONICET PIP 0022 to Dra. F. Arrighetti, and grant from FCNyM-UNLP N° 738 . We appreciate the kind help of Dr. Alfredo Castro-Vazquez for interpreting the digestive gland histology. We are grateful to Dr. Fernando Spaccesi, Roberto Jensen, and Dr. Hernán Benitez for their help in sample collection, to Sergio Mijailovsky for chemical analysis and to Norma Tedesco for language revision. We also thank Mario Ramos and Luciana De Tezanos for graphic design assistance. This paper is Scientific Contribution N° 1010 of the Institute of Limnology “Dr. Raúl A. Ringuelet” (ILPLA, CCT-La Plata CONICET, UNLP).

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10.1016/j.aquatox.2017.11.014

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@article{c77473a82c814ee8ad6bf095fcc4fcd8,

title = "Differential response between histological and biochemical biomarkers in the apple snail Pomacea canaliculata (Gasteropoda: Amullariidae) exposed to cypermethrin",

abstract = "To develop effective programs to monitor water quality is necessary to identify sensitive biomarkers in indicator species. The aim of this study was to evaluate different biomarkers in the apple snail Pomacea canaliculata exposed to the insecticide Cypermethrin (CYP). Adult male and female snails were exposed to sublethal CYP concentrations (10, 25 and 100 μg l−1) for 1, 4, 7 and 14 days. The recovery of the exposed snails was also studied by a post-exposure assay. The activities of the enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST), the levels of lipid peroxidation (LPO) and protein oxidation (PC) in digestive gland and gills were studied as biomarkers of exposure. Histopathological changes in target tissues were also evaluated. In digestive gland, CYP caused a significant increase in SOD, CAT and GST activities compared to control (p < 0.05) as well as in LPO and PC levels (p < 0.05). However, such biochemical effects were neither concentration nor time dependent. Histopatological changes were observed in the exposed groups, such as an increase in the number of basophilic cells, hemocytic infiltration and epithelia atrophy. Additionally, a positive correlation between the surface occupied by pigmented corpuscles and CYP concentrations was observed at all exposure periods. Gills showed greater sensitivity to oxidative damage than digestive gland. CYP caused an acute toxic effect in LPO levels in this respiratory organ. The gill filament of exposed snails, exhibited a reduction or loss of cilia, vacuolization of the columnar cells and an increase in haemocyte content irrespective of the concentration. High concentrations of CYP caused disruptions in the columnar muscle fibers. In general, snails did not show an improvement in their basal state during post-exposure treatment. Apparently, males and females do not have differential sensitivity to the pesticide. The results of this study suggest that histopathological changes are the most sensitive time- and dose-dependent biomarkers of toxicity induced by CYP in P. canaliculata.",

author = "F. Arrighetti and E. Ambrosio and M. Astiz and Cap{\'i}tulo, \{A. Rodrigues\} and S. Lavar{\'i}as",

note = "Funding Information: This work was partially supported by grants from Agencia Nacional de Promoci{\'o}n Cient{\'i}fica y T{\'e}cnica, Argentina ( PICT 2014-0810 and PICT 2015-0669 ) to Dr. A. Rodrigues Cap{\'i}tulo and Dr. F. Arrighetti respectively, and grant from CONICET PIP 0022 to Dra. F. Arrighetti, and grant from FCNyM-UNLP N° 738 . We appreciate the kind help of Dr. Alfredo Castro-Vazquez for interpreting the digestive gland histology. We are grateful to Dr. Fernando Spaccesi, Roberto Jensen, and Dr. Hern{\'a}n Benitez for their help in sample collection, to Sergio Mijailovsky for chemical analysis and to Norma Tedesco for language revision. We also thank Mario Ramos and Luciana De Tezanos for graphic design assistance. This paper is Scientific Contribution N° 1010 of the Institute of Limnology “Dr. Ra{\'u}l A. Ringuelet” (ILPLA, CCT-La Plata CONICET, UNLP). Publisher Copyright: {\textcopyright} 2017 Elsevier B.V. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2018",

month = jan,

doi = "10.1016/j.aquatox.2017.11.014",

language = "English",

volume = "194",

pages = "140--151",

journal = "Aquatic Toxicology",

issn = "0166-445X",

publisher = "Elsevier B.V.",

}

Differential response between histological and biochemical biomarkers in the apple snail Pomacea canaliculata (Gasteropoda: Amullariidae) exposed to cypermethrin. / Arrighetti, F.; Ambrosio, E.; Astiz, M. et al.
in: Aquatic Toxicology, Jahrgang 194, 01.2018, S. 140-151.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - Differential response between histological and biochemical biomarkers in the apple snail Pomacea canaliculata (Gasteropoda: Amullariidae) exposed to cypermethrin

AU - Arrighetti, F.

AU - Ambrosio, E.

AU - Astiz, M.

AU - Capítulo, A. Rodrigues

AU - Lavarías, S.

N1 - Funding Information: This work was partially supported by grants from Agencia Nacional de Promoción Científica y Técnica, Argentina ( PICT 2014-0810 and PICT 2015-0669 ) to Dr. A. Rodrigues Capítulo and Dr. F. Arrighetti respectively, and grant from CONICET PIP 0022 to Dra. F. Arrighetti, and grant from FCNyM-UNLP N° 738 . We appreciate the kind help of Dr. Alfredo Castro-Vazquez for interpreting the digestive gland histology. We are grateful to Dr. Fernando Spaccesi, Roberto Jensen, and Dr. Hernán Benitez for their help in sample collection, to Sergio Mijailovsky for chemical analysis and to Norma Tedesco for language revision. We also thank Mario Ramos and Luciana De Tezanos for graphic design assistance. This paper is Scientific Contribution N° 1010 of the Institute of Limnology “Dr. Raúl A. Ringuelet” (ILPLA, CCT-La Plata CONICET, UNLP). Publisher Copyright: © 2017 Elsevier B.V. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2018/1

Y1 - 2018/1

N2 - To develop effective programs to monitor water quality is necessary to identify sensitive biomarkers in indicator species. The aim of this study was to evaluate different biomarkers in the apple snail Pomacea canaliculata exposed to the insecticide Cypermethrin (CYP). Adult male and female snails were exposed to sublethal CYP concentrations (10, 25 and 100 μg l−1) for 1, 4, 7 and 14 days. The recovery of the exposed snails was also studied by a post-exposure assay. The activities of the enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST), the levels of lipid peroxidation (LPO) and protein oxidation (PC) in digestive gland and gills were studied as biomarkers of exposure. Histopathological changes in target tissues were also evaluated. In digestive gland, CYP caused a significant increase in SOD, CAT and GST activities compared to control (p < 0.05) as well as in LPO and PC levels (p < 0.05). However, such biochemical effects were neither concentration nor time dependent. Histopatological changes were observed in the exposed groups, such as an increase in the number of basophilic cells, hemocytic infiltration and epithelia atrophy. Additionally, a positive correlation between the surface occupied by pigmented corpuscles and CYP concentrations was observed at all exposure periods. Gills showed greater sensitivity to oxidative damage than digestive gland. CYP caused an acute toxic effect in LPO levels in this respiratory organ. The gill filament of exposed snails, exhibited a reduction or loss of cilia, vacuolization of the columnar cells and an increase in haemocyte content irrespective of the concentration. High concentrations of CYP caused disruptions in the columnar muscle fibers. In general, snails did not show an improvement in their basal state during post-exposure treatment. Apparently, males and females do not have differential sensitivity to the pesticide. The results of this study suggest that histopathological changes are the most sensitive time- and dose-dependent biomarkers of toxicity induced by CYP in P. canaliculata.

AB - To develop effective programs to monitor water quality is necessary to identify sensitive biomarkers in indicator species. The aim of this study was to evaluate different biomarkers in the apple snail Pomacea canaliculata exposed to the insecticide Cypermethrin (CYP). Adult male and female snails were exposed to sublethal CYP concentrations (10, 25 and 100 μg l−1) for 1, 4, 7 and 14 days. The recovery of the exposed snails was also studied by a post-exposure assay. The activities of the enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST), the levels of lipid peroxidation (LPO) and protein oxidation (PC) in digestive gland and gills were studied as biomarkers of exposure. Histopathological changes in target tissues were also evaluated. In digestive gland, CYP caused a significant increase in SOD, CAT and GST activities compared to control (p < 0.05) as well as in LPO and PC levels (p < 0.05). However, such biochemical effects were neither concentration nor time dependent. Histopatological changes were observed in the exposed groups, such as an increase in the number of basophilic cells, hemocytic infiltration and epithelia atrophy. Additionally, a positive correlation between the surface occupied by pigmented corpuscles and CYP concentrations was observed at all exposure periods. Gills showed greater sensitivity to oxidative damage than digestive gland. CYP caused an acute toxic effect in LPO levels in this respiratory organ. The gill filament of exposed snails, exhibited a reduction or loss of cilia, vacuolization of the columnar cells and an increase in haemocyte content irrespective of the concentration. High concentrations of CYP caused disruptions in the columnar muscle fibers. In general, snails did not show an improvement in their basal state during post-exposure treatment. Apparently, males and females do not have differential sensitivity to the pesticide. The results of this study suggest that histopathological changes are the most sensitive time- and dose-dependent biomarkers of toxicity induced by CYP in P. canaliculata.

UR - https://www.scopus.com/pages/publications/85034807967

U2 - 10.1016/j.aquatox.2017.11.014

DO - 10.1016/j.aquatox.2017.11.014

M3 - Journal articles

C2 - 29179149

AN - SCOPUS:85034807967

SN - 0166-445X

VL - 194

SP - 140

EP - 151

JO - Aquatic Toxicology

JF - Aquatic Toxicology

ER -

Differential response between histological and biochemical biomarkers in the apple snail Pomacea canaliculata (Gasteropoda: Amullariidae) exposed to cypermethrin

Abstract

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