Diagnosis of epidermolysis bullosa acquisita: Multicentre comparison of different assays for serum anti-type VII collagen reactivity

Maike Marleen Holtsche; Nina Van Beek; Takashi Hashimoto; Giovanni Di Zenzo; Detlef Zillikens; Catherine Prost-Squarcioni; Matthias Titeux; Alain Hovnanian; Enno Schmidt; Stephanie Goletz

doi:10.2340/00015555-3774

Diagnosis of epidermolysis bullosa acquisita: Multicentre comparison of different assays for serum anti-type VII collagen reactivity

Maike Marleen Holtsche, Nina Van Beek, Takashi Hashimoto, Giovanni Di Zenzo, Detlef Zillikens, Catherine Prost-Squarcioni, Matthias Titeux, Alain Hovnanian, Enno Schmidt, Stephanie Goletz^*

^*Korrespondierende/r Autor/-in für diese Arbeit

11 Zitate (Scopus)

Abstract

Epidermolysis bullosa acquisita is a pemphigoid disease characterized by autoantibodies against type VII collagen. This study compared the sensitivity and specificity of 6 diagnostic assays: type VII collagen noncollagenous domains enzyme-linked immunoassay (NC1/2 ELISA) (MBL, Nagoya, Japan); type VII collagen NC1 ELISA (Euroimmun, Lübeck, Germany); indirect immunofluorescence (IF) microscopy test based on the expression of recombinant NC1 in a human cell line (NC1 BIOCHIP®; Euroimmun); full-length recombinant type VII collagen ELISA; immunoblotting with full-length type VII collagen in the extract of human dermis; and immunoblotting with recombinant NC1. Immunoblotting with recombinant NC1 showed a sensitivity of 93.1% and specificity of 100%, followed by NC1 BIOCHIP® (sensitivity, 89.1%; specificity, 100%), immunoblotting with human dermis (sensitivity, 87.1%; specificity 100%), NC1-ELISA (sensitivity 82.2%; specificity 98.6%), NC1/NC2 ELISA (sensitivity 88.1%; specificity 93.3%), and full-length type VII collagen ELISA (sensitivity 80.2%; specificity 93.8%).

Originalsprache	Englisch
Aufsatznummer	adv00420
Zeitschrift	Acta Dermato-Venereologica
Jahrgang	101
Ausgabenummer	3
Seiten (von - bis)	adv00420
ISSN	0001-5555
DOIs	https://doi.org/10.2340/00015555-3774
Publikationsstatus	Veröffentlicht - 2021

Fördermittel

This work was supported by grants from the Deutsche Forschungsgemeinschaft through CRU 303 Pemphigoid Diseases and Excellence Cluster Precision Medicine in Chronic Inflammation (EXC 2167). Type VII collagen NC1/2 ELISA (MBL, Nagoya, Japan) was purchased for a reduced price, type VII collagen NC1 ELISA and BIOCHIP® (Euroimmun, Lübeck, Germany) were provided free of charge. The authors thank Sarah Gaugel, L?beck, and Pierre Bonnesoeur, Paris, for excellent technical assistance. IDI-IRCCS and the Department of Dermatology, University of L?beck, are healthcare providers of the European Reference Network (ERN)-Skin. This work was supported by grants from the Deutsche Forschungsgemeinschaft through CRU 303 Pemphigoid Diseases and Excellence Cluster Precision Medicine in Chronic Inflammation (EXC 2167). Type VII collagen NC1/2 ELISA (MBL, Nagoya, Japan) was purchased for a reduced price, type VII collagen NC1 ELISA and BIOCHIP? (Euroimmun, L?beck, Germany) were provided free of charge. DZ and ES have a scientific cooperation with Euroimmun. The other authors have declared no conflicts of interest.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen
SDG 6 – Sauberes Wasser und sanitäre Einrichtungen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)
Zentren: Center for Research on Inflammation of the Skin (CRIS)

DFG-Fachsystematik

2.21-05 Immunologie
2.22-19 Dermatologie

Dokumente

10.2340/00015555-3774

Weitere Links

Link to publication in Scopus

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EXC 2167: Präzisionsmedizin für Chronische Entzündungserkrankungen (PMI)
Schreiber, S. (Sprecher*in), Baines, J. F. (Beteiligte*r Wissenschaftler*in), Bosch, T. C. G. (Beteiligte*r Wissenschaftler*in), Buyx, A. (Beteiligte*r Wissenschaftler*in), Erdmann, J. (Beteiligte*r Wissenschaftler*in), Franke, A. (Beteiligte*r Wissenschaftler*in), Huber, R. (Beteiligte*r Wissenschaftler*in), Klein, C. (Beteiligte*r Wissenschaftler*in), Köhl, J. (Beteiligte*r Wissenschaftler*in), König, I. R. (Beteiligte*r Wissenschaftler*in), Lange, C. (Beteiligte*r Wissenschaftler*in), Laudes, M. (Beteiligte*r Wissenschaftler*in), Lieb, W. (Beteiligte*r Wissenschaftler*in), Ludwig, R. (Beteiligte*r Wissenschaftler*in), Nebel, A. (Beteiligte*r Wissenschaftler*in), Niemann, S. (Sprecher*in), Rabe, K. F. (Beteiligte*r Wissenschaftler*in), Riemekasten, G. (Sprecher*in), Rose-John, S. (Beteiligte*r Wissenschaftler*in), Rosenstiel, P. C. (Beteiligte*r Wissenschaftler*in), Schulenburg, H. (Beteiligte*r Wissenschaftler*in), Schwarz, K. (Beteiligte*r Wissenschaftler*in), Traulsen, A. (Beteiligte*r Wissenschaftler*in), Weidinger, S. (Beteiligte*r Wissenschaftler*in) & Zillikens, D. (Beteiligte*r Wissenschaftler*in)
01.01.19 → 31.12.32
Projekt: DFG Verbundprojekte › Exzellenzcluster

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Holtsche, M. M., Van Beek, N., Hashimoto, T., Di Zenzo, G., Zillikens, D., Prost-Squarcioni, C., Titeux, M., Hovnanian, A., Schmidt, E., & Goletz, S. (2021). Diagnosis of epidermolysis bullosa acquisita: Multicentre comparison of different assays for serum anti-type VII collagen reactivity. Acta Dermato-Venereologica, 101(3), adv00420. Artikel adv00420. https://doi.org/10.2340/00015555-3774

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title = "Diagnosis of epidermolysis bullosa acquisita: Multicentre comparison of different assays for serum anti-type VII collagen reactivity",

abstract = "Epidermolysis bullosa acquisita is a pemphigoid disease characterized by autoantibodies against type VII collagen. This study compared the sensitivity and specificity of 6 diagnostic assays: type VII collagen noncollagenous domains enzyme-linked immunoassay (NC1/2 ELISA) (MBL, Nagoya, Japan); type VII collagen NC1 ELISA (Euroimmun, L{\"u}beck, Germany); indirect immunofluorescence (IF) microscopy test based on the expression of recombinant NC1 in a human cell line (NC1 BIOCHIP{\textregistered}; Euroimmun); full-length recombinant type VII collagen ELISA; immunoblotting with full-length type VII collagen in the extract of human dermis; and immunoblotting with recombinant NC1. Immunoblotting with recombinant NC1 showed a sensitivity of 93.1\% and specificity of 100\%, followed by NC1 BIOCHIP{\textregistered} (sensitivity, 89.1\%; specificity, 100\%), immunoblotting with human dermis (sensitivity, 87.1\%; specificity 100\%), NC1-ELISA (sensitivity 82.2\%; specificity 98.6\%), NC1/NC2 ELISA (sensitivity 88.1\%; specificity 93.3\%), and full-length type VII collagen ELISA (sensitivity 80.2\%; specificity 93.8\%).",

author = "Holtsche, \{Maike Marleen\} and \{Van Beek\}, Nina and Takashi Hashimoto and \{Di Zenzo\}, Giovanni and Detlef Zillikens and Catherine Prost-Squarcioni and Matthias Titeux and Alain Hovnanian and Enno Schmidt and Stephanie Goletz",

year = "2021",

doi = "10.2340/00015555-3774",

language = "English",

volume = "101",

pages = "adv00420",

journal = "Acta Dermato-Venereologica",

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Holtsche, MM , Van Beek, N, Hashimoto, T, Di Zenzo, G, Zillikens, D, Prost-Squarcioni, C, Titeux, M, Hovnanian, A, Schmidt, E & Goletz, S 2021, 'Diagnosis of epidermolysis bullosa acquisita: Multicentre comparison of different assays for serum anti-type VII collagen reactivity', Acta Dermato-Venereologica, Jg. 101, Nr. 3, adv00420, S. adv00420. https://doi.org/10.2340/00015555-3774

Diagnosis of epidermolysis bullosa acquisita: Multicentre comparison of different assays for serum anti-type VII collagen reactivity. / Holtsche, Maike Marleen ; Van Beek, Nina; Hashimoto, Takashi et al.
in: Acta Dermato-Venereologica, Jahrgang 101, Nr. 3, adv00420, 2021, S. adv00420.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - Diagnosis of epidermolysis bullosa acquisita: Multicentre comparison of different assays for serum anti-type VII collagen reactivity

AU - Holtsche, Maike Marleen

AU - Van Beek, Nina

AU - Hashimoto, Takashi

AU - Di Zenzo, Giovanni

AU - Zillikens, Detlef

AU - Prost-Squarcioni, Catherine

AU - Titeux, Matthias

AU - Hovnanian, Alain

AU - Schmidt, Enno

AU - Goletz, Stephanie

PY - 2021

Y1 - 2021

N2 - Epidermolysis bullosa acquisita is a pemphigoid disease characterized by autoantibodies against type VII collagen. This study compared the sensitivity and specificity of 6 diagnostic assays: type VII collagen noncollagenous domains enzyme-linked immunoassay (NC1/2 ELISA) (MBL, Nagoya, Japan); type VII collagen NC1 ELISA (Euroimmun, Lübeck, Germany); indirect immunofluorescence (IF) microscopy test based on the expression of recombinant NC1 in a human cell line (NC1 BIOCHIP®; Euroimmun); full-length recombinant type VII collagen ELISA; immunoblotting with full-length type VII collagen in the extract of human dermis; and immunoblotting with recombinant NC1. Immunoblotting with recombinant NC1 showed a sensitivity of 93.1% and specificity of 100%, followed by NC1 BIOCHIP® (sensitivity, 89.1%; specificity, 100%), immunoblotting with human dermis (sensitivity, 87.1%; specificity 100%), NC1-ELISA (sensitivity 82.2%; specificity 98.6%), NC1/NC2 ELISA (sensitivity 88.1%; specificity 93.3%), and full-length type VII collagen ELISA (sensitivity 80.2%; specificity 93.8%).

AB - Epidermolysis bullosa acquisita is a pemphigoid disease characterized by autoantibodies against type VII collagen. This study compared the sensitivity and specificity of 6 diagnostic assays: type VII collagen noncollagenous domains enzyme-linked immunoassay (NC1/2 ELISA) (MBL, Nagoya, Japan); type VII collagen NC1 ELISA (Euroimmun, Lübeck, Germany); indirect immunofluorescence (IF) microscopy test based on the expression of recombinant NC1 in a human cell line (NC1 BIOCHIP®; Euroimmun); full-length recombinant type VII collagen ELISA; immunoblotting with full-length type VII collagen in the extract of human dermis; and immunoblotting with recombinant NC1. Immunoblotting with recombinant NC1 showed a sensitivity of 93.1% and specificity of 100%, followed by NC1 BIOCHIP® (sensitivity, 89.1%; specificity, 100%), immunoblotting with human dermis (sensitivity, 87.1%; specificity 100%), NC1-ELISA (sensitivity 82.2%; specificity 98.6%), NC1/NC2 ELISA (sensitivity 88.1%; specificity 93.3%), and full-length type VII collagen ELISA (sensitivity 80.2%; specificity 93.8%).

UR - https://www.scopus.com/pages/publications/85103130698

U2 - 10.2340/00015555-3774

DO - 10.2340/00015555-3774

M3 - Journal articles

C2 - 33686442

AN - SCOPUS:85103130698

SN - 0001-5555

VL - 101

SP - adv00420

JO - Acta Dermato-Venereologica

JF - Acta Dermato-Venereologica

IS - 3

M1 - adv00420

ER -

Diagnosis of epidermolysis bullosa acquisita: Multicentre comparison of different assays for serum anti-type VII collagen reactivity

Abstract

Fördermittel

UN SDGs

Strategische Forschungsbereiche und Zentren

DFG-Fachsystematik

Dokumente

Weitere Links

Fingerprint

Projekte

EXC 2167: Präzisionsmedizin für Chronische Entzündungserkrankungen (PMI)

Zitieren