Determination of transforming growth factor β2 in human blood samples by ELISA

Christof H. Szymkowiak; Ingrid Mons; Wolfgang L. Gross; Jörn Kekow

doi:10.1016/0022-1759(95)00098-U

Determination of transforming growth factor β₂ in human blood samples by ELISA

Christof H. Szymkowiak, Ingrid Mons, Wolfgang L. Gross, Jörn Kekow^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Klinik für Rheumatologie und klinische Immunologie

25 Zitate (Scopus)

Abstract

Transforming growth factors β (TGFβs) show pleotrophic functions in vitro and in vivo. Biological effects depend on the cell type, the TGFβ isoform, and the availability of active TGFβ. Bioassays for TGFβ have not proven to be reliable tools in the measurement of TGFβ in human blood samples. Previously described sandwich ELISAs for measuring TGFβ are limited to single TGFβ isoforms and have not been evaluated for use in human sera or plasma. We describe a simple procedure to quantitate not only TGFβ₁ but also TGFβ₂ in human blood samples using commercially available antibodies. The sensitivity of the TGFβ₂ ELISA was 11 pg/ml. There was no crossreactivity between the TGFβ₁ and TGFβ₃ isoforms. High concentrations of TGFβ₁ and TGFβ₃ in spiked samples did not interfere with TGFβ₂ determination. TGFβ₂ recovery was highest in EDTA plasma (> 88%), and the intra-and interassay variance was 6% and 8.5% respectively. Applying the ELISA to human plasma specimens a significant percentage of TGFβ₂ (3.7 ± 0.8 ng/ml) was found (about 50% of the TGFβ₁ content).

Originalsprache	Englisch
Zeitschrift	Journal of Immunological Methods
Jahrgang	184
Ausgabenummer	2
Seiten (von - bis)	263-271
Seitenumfang	9
ISSN	0022-1759
DOIs	https://doi.org/10.1016/0022-1759(95)00098-U
Publikationsstatus	Veröffentlicht - 18.08.1995

Fördermittel

This work was supported by grants from the Bundesministerium fiir Forschung und Technolo-gie (autoimmunity grant, 01 KD 89030), Bonn, and from the Deutsche Forschungsgemeinschaft (grant GR 607/7-l), Bonn. Funds were also provided by the Verein zur Forderung der Er-forschung und Beklmpfung rheumatischer Erkrankungen e.V., Bad Bramstedt, Germany.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

Dokumente

10.1016/0022-1759(95)00098-U

Weitere Links

Link to publication in Scopus

Zitieren

@article{8eadb6ffdb1944588c11f20d9e947505,

title = "Determination of transforming growth factor β2 in human blood samples by ELISA",

abstract = "Transforming growth factors β (TGFβs) show pleotrophic functions in vitro and in vivo. Biological effects depend on the cell type, the TGFβ isoform, and the availability of active TGFβ. Bioassays for TGFβ have not proven to be reliable tools in the measurement of TGFβ in human blood samples. Previously described sandwich ELISAs for measuring TGFβ are limited to single TGFβ isoforms and have not been evaluated for use in human sera or plasma. We describe a simple procedure to quantitate not only TGFβ1 but also TGFβ2 in human blood samples using commercially available antibodies. The sensitivity of the TGFβ2 ELISA was 11 pg/ml. There was no crossreactivity between the TGFβ1 and TGFβ3 isoforms. High concentrations of TGFβ1 and TGFβ3 in spiked samples did not interfere with TGFβ2 determination. TGFβ2 recovery was highest in EDTA plasma (> 88\%), and the intra-and interassay variance was 6\% and 8.5\% respectively. Applying the ELISA to human plasma specimens a significant percentage of TGFβ2 (3.7 ± 0.8 ng/ml) was found (about 50\% of the TGFβ1 content).",

author = "Szymkowiak, \{Christof H.\} and Ingrid Mons and Gross, \{Wolfgang L.\} and J{\"o}rn Kekow",

note = "Funding Information: This work was supported by grants from the Bundesministerium fiir Forschung und Technolo-gie (autoimmunity grant, 01 KD 89030), Bonn, and from the Deutsche Forschungsgemeinschaft (grant GR 607/7-l), Bonn. Funds were also provided by the Verein zur Forderung der Er-forschung und Beklmpfung rheumatischer Erkrankungen e.V., Bad Bramstedt, Germany. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.",

year = "1995",

month = aug,

day = "18",

doi = "10.1016/0022-1759(95)00098-U",

language = "English",

volume = "184",

pages = "263--271",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Determination of transforming growth factor β2 in human blood samples by ELISA

AU - Szymkowiak, Christof H.

AU - Mons, Ingrid

AU - Gross, Wolfgang L.

AU - Kekow, Jörn

N1 - Funding Information: This work was supported by grants from the Bundesministerium fiir Forschung und Technolo-gie (autoimmunity grant, 01 KD 89030), Bonn, and from the Deutsche Forschungsgemeinschaft (grant GR 607/7-l), Bonn. Funds were also provided by the Verein zur Forderung der Er-forschung und Beklmpfung rheumatischer Erkrankungen e.V., Bad Bramstedt, Germany. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.

PY - 1995/8/18

Y1 - 1995/8/18

N2 - Transforming growth factors β (TGFβs) show pleotrophic functions in vitro and in vivo. Biological effects depend on the cell type, the TGFβ isoform, and the availability of active TGFβ. Bioassays for TGFβ have not proven to be reliable tools in the measurement of TGFβ in human blood samples. Previously described sandwich ELISAs for measuring TGFβ are limited to single TGFβ isoforms and have not been evaluated for use in human sera or plasma. We describe a simple procedure to quantitate not only TGFβ1 but also TGFβ2 in human blood samples using commercially available antibodies. The sensitivity of the TGFβ2 ELISA was 11 pg/ml. There was no crossreactivity between the TGFβ1 and TGFβ3 isoforms. High concentrations of TGFβ1 and TGFβ3 in spiked samples did not interfere with TGFβ2 determination. TGFβ2 recovery was highest in EDTA plasma (> 88%), and the intra-and interassay variance was 6% and 8.5% respectively. Applying the ELISA to human plasma specimens a significant percentage of TGFβ2 (3.7 ± 0.8 ng/ml) was found (about 50% of the TGFβ1 content).

AB - Transforming growth factors β (TGFβs) show pleotrophic functions in vitro and in vivo. Biological effects depend on the cell type, the TGFβ isoform, and the availability of active TGFβ. Bioassays for TGFβ have not proven to be reliable tools in the measurement of TGFβ in human blood samples. Previously described sandwich ELISAs for measuring TGFβ are limited to single TGFβ isoforms and have not been evaluated for use in human sera or plasma. We describe a simple procedure to quantitate not only TGFβ1 but also TGFβ2 in human blood samples using commercially available antibodies. The sensitivity of the TGFβ2 ELISA was 11 pg/ml. There was no crossreactivity between the TGFβ1 and TGFβ3 isoforms. High concentrations of TGFβ1 and TGFβ3 in spiked samples did not interfere with TGFβ2 determination. TGFβ2 recovery was highest in EDTA plasma (> 88%), and the intra-and interassay variance was 6% and 8.5% respectively. Applying the ELISA to human plasma specimens a significant percentage of TGFβ2 (3.7 ± 0.8 ng/ml) was found (about 50% of the TGFβ1 content).

UR - https://www.scopus.com/pages/publications/0029056184

U2 - 10.1016/0022-1759(95)00098-U

DO - 10.1016/0022-1759(95)00098-U

M3 - Journal articles

C2 - 7658028

AN - SCOPUS:0029056184

SN - 0022-1759

VL - 184

SP - 263

EP - 271

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -