Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system

Marjeta Hofko; Alexander Mischnik; Martin Kaase; Stefan Zimmermann; Alexander H. Dalpke

doi:10.1128/JCM.00373-14

Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system

Marjeta Hofko, Alexander Mischnik, Martin Kaase, Stefan Zimmermann, Alexander H. Dalpke^*

^*Korrespondierende/r Autor/-in für diese Arbeit

29 Zitate (Scopus)

Abstract

We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.

Originalsprache	Englisch
Zeitschrift	Journal of Clinical Microbiology
Jahrgang	52
Ausgabenummer	5
Seiten (von - bis)	1701-1704
Seitenumfang	4
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.00373-14
Publikationsstatus	Veröffentlicht - 01.01.2014

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

Dokumente

10.1128/JCM.00373-14

Weitere Links

Link to publication in Scopus

Zitieren

@article{aa63fb48a4c34201bc888e800eb0e3df,

title = "Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system",

abstract = "We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.",

author = "Marjeta Hofko and Alexander Mischnik and Martin Kaase and Stefan Zimmermann and Dalpke, \{Alexander H.\}",

year = "2014",

month = jan,

day = "1",

doi = "10.1128/JCM.00373-14",

language = "English",

volume = "52",

pages = "1701--1704",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "5",

}

TY - JOUR

T1 - Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system

AU - Hofko, Marjeta

AU - Mischnik, Alexander

AU - Kaase, Martin

AU - Zimmermann, Stefan

AU - Dalpke, Alexander H.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.

AB - We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.

UR - https://www.scopus.com/pages/publications/84899511321

U2 - 10.1128/JCM.00373-14

DO - 10.1128/JCM.00373-14

M3 - Journal articles

C2 - 24523474

AN - SCOPUS:84899511321

SN - 0095-1137

VL - 52

SP - 1701

EP - 1704

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system

Abstract

UN SDGs

Strategische Forschungsbereiche und Zentren

Dokumente

Weitere Links

Fingerprint

Zitieren