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Abstract
Twophotonmicroscopy has substantially advanced our understanding of cellular dynamics in the immune system. Cell migration can now be imaged in real time in the living animal. Strikingly, the migration of naive lymphocytes in secondary lymphoid tissue appears predominantly random. It is unclear, however, whether directed migration may escape detection in this random background. Using a combination of mathematical modeling and experimental data, we investigate the extent to which modern twophoton imaging can rule out biologically relevant directed migration. For naive T cells migrating in uninfected lymph nodes (LNs) at average 3D speeds of around 18 μm/min, we rule out uniform directed migration of more than 1.7 μm/min at the 95% confidence level, confirming that T cell migration is indeed mostly random on a timescale of minutes. To investigate whether this finding still holds for longer timescales, we use a 3D simulation of the naive T cell LN transit. A pure random walk predicts a transit time of around 16 h, which is in good agreement with experimental results. A directional bias of only 0.5 μm/min  less than 3% of the cell speed  would already accelerate the transit twofold. These results jointly strengthen the random walk analogy for naive T cell migration in LNs, but they also emphasize that very small deviations from random migration can still be important. Our methods are applicable to cells of any type and can be used to reanalyze existing datasets.
Originalsprache  Englisch 

Zeitschrift  Proceedings of the National Academy of Sciences of the United States of America 
Jahrgang  108 
Ausgabenummer  30 
Seiten (von  bis)  1240112406 
Seitenumfang  6 
ISSN  00278424 
DOIs  
Publikationsstatus  Veröffentlicht  26.07.2011 
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 1 Abgeschlossen

Graduiertenschule GSC 235: Informatik in Medizin und Lebenswissenschaften
Schweikard, A., Anders, S., Barkhausen, J., Buzug, T., Erdmann, J., Fischer, B., Fischer, S., Habermann, J. K., Hartmann, E., Hilgenfeld, R., Hofmann, U., Klein, C., Kruse, C., Marshall, L., Martinetz, T., Mertins, A., Münte, T., Oltmanns, K., Schneider, S., Schunkert, H., Sczakiel, G., Tronnier, V. M., Vogel, A., Westermann, J. & Zillikens, D.
01.11.07 → 31.12.14
Projekt: DFGProjekte › DFGVerbundforschung: Graduiertenkollegs