Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from Sulfolobus acidocaldarius

Heiko Bönisch; Christian L. Schmidt; Günter Schäfer; Rudolf Ladenstein

doi:10.1107/S0907444900002456

Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from Sulfolobus acidocaldarius

Heiko Bönisch^*, Christian L. Schmidt, Günter Schäfer, Rudolf Ladenstein

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Biochemie

Karolinska Institutet

2 Zitate (Scopus)

Abstract

An archaeal Rieske iron-sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe-2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P6₁ or P6₅, with unit-cell parameters a = b = 80.19, c = 75.69 Å. A complete data set has been collected to 1.64 Å resolution at 100 K.

Originalsprache	Englisch
Zeitschrift	Acta Crystallographica Section D: Biological Crystallography
Jahrgang	56
Ausgabenummer	5
Seiten (von - bis)	643-644
Seitenumfang	2
ISSN	0907-4449
DOIs	https://doi.org/10.1107/S0907444900002456
Publikationsstatus	Veröffentlicht - 2000

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title = "Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from Sulfolobus acidocaldarius",

abstract = "An archaeal Rieske iron-sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe-2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P61 or P65, with unit-cell parameters a = b = 80.19, c = 75.69 {\AA}. A complete data set has been collected to 1.64 {\AA} resolution at 100 K.",

author = "Heiko B{\"o}nisch and Schmidt, \{Christian L.\} and G{\"u}nter Sch{\"a}fer and Rudolf Ladenstein",

year = "2000",

doi = "10.1107/S0907444900002456",

language = "English",

volume = "56",

pages = "643--644",

journal = "Acta Crystallographica Section D: Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

number = "5",

}

Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from Sulfolobus acidocaldarius. / Bönisch, Heiko; Schmidt, Christian L.; Schäfer, Günter et al.
in: Acta Crystallographica Section D: Biological Crystallography, Jahrgang 56, Nr. 5, 2000, S. 643-644.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from Sulfolobus acidocaldarius

AU - Bönisch, Heiko

AU - Schmidt, Christian L.

AU - Schäfer, Günter

AU - Ladenstein, Rudolf

PY - 2000

Y1 - 2000

N2 - An archaeal Rieske iron-sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe-2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P61 or P65, with unit-cell parameters a = b = 80.19, c = 75.69 Å. A complete data set has been collected to 1.64 Å resolution at 100 K.

AB - An archaeal Rieske iron-sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe-2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P61 or P65, with unit-cell parameters a = b = 80.19, c = 75.69 Å. A complete data set has been collected to 1.64 Å resolution at 100 K.

UR - https://www.scopus.com/pages/publications/12944277037

U2 - 10.1107/S0907444900002456

DO - 10.1107/S0907444900002456

M3 - Journal articles

C2 - 10771436

AN - SCOPUS:12944277037

SN - 0907-4449

VL - 56

SP - 643

EP - 644

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 5

ER -

Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from Sulfolobus acidocaldarius

Abstract

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