TY - JOUR
T1 - Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffinembedded bone tissue
AU - Saraji, Alireza
AU - Offermann, Anne
AU - Stegmann-Frehse, Janine
AU - Hempel, Katharina
AU - Kang, Duan
AU - Krupar, Rosemarie
AU - Watermann, Christian
AU - Jonigk, Danny
AU - Kuhnel, Mark Philipp
AU - Kirfel, Jutta
AU - Perner, Sven
AU - Sailer, Verena
N1 - Publisher Copyright:
© 2021 Saraji et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2021/9
Y1 - 2021/9
N2 - With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was long thought that transcriptome analysis will not be feasible from decalcified formalin-fixed, paraffin-embedded (DFFPE) in a large manner. Here we demonstrate that mRNA extraction from DFFPE is feasible, quick, robust and reproducible and that decalcification does not hamper subsequent gene expression analysis. This might assist in implementing transcriptome analysis from DFFPE into every day practice.
AB - With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was long thought that transcriptome analysis will not be feasible from decalcified formalin-fixed, paraffin-embedded (DFFPE) in a large manner. Here we demonstrate that mRNA extraction from DFFPE is feasible, quick, robust and reproducible and that decalcification does not hamper subsequent gene expression analysis. This might assist in implementing transcriptome analysis from DFFPE into every day practice.
UR - http://www.scopus.com/inward/record.url?scp=85115050138&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0257416
DO - 10.1371/journal.pone.0257416
M3 - Journal articles
C2 - 34529723
AN - SCOPUS:85115050138
VL - 16
JO - PLoS ONE
JF - PLoS ONE
IS - 9 September
M1 - e0257416
ER -
