Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Jeanette Erdmann; Sabine Hassfeld; Heike Kallisch; Eckart Fleck; Vera Regitz-Zagrosek

doi:10.1006/bbrc.1998.8311

Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Jeanette Erdmann^*, Sabine Hassfeld, Heike Kallisch, Eckart Fleck, Vera Regitz-Zagrosek

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Kardiogenetik

Charite - Universitatsmedizin Berlin

10 Zitate (Scopus)

Abstract

To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

Originalsprache	Englisch
Zeitschrift	Biochemical and Biophysical Research Communications
Jahrgang	244
Ausgabenummer	2
Seiten (von - bis)	494-497
Seitenumfang	4
ISSN	0006-291X
DOIs	https://doi.org/10.1006/bbrc.1998.8311
Publikationsstatus	Veröffentlicht - 17.03.1998

Fördermittel

This study was supported in part by a grant of the Verbund Klin-ische Pharmakologie Berlin/Brandenburg.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen
SDG 5 – Gender Equality

Dokumente

10.1006/bbrc.1998.8311

Weitere Links

Link to publication in Scopus

Zitieren

@article{0042a37166704fd2b408e37b18ba0504,

title = "Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene",

abstract = "To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.",

author = "Jeanette Erdmann and Sabine Hassfeld and Heike Kallisch and Eckart Fleck and Vera Regitz-Zagrosek",

year = "1998",

month = mar,

day = "17",

doi = "10.1006/bbrc.1998.8311",

language = "English",

volume = "244",

pages = "494--497",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

AU - Erdmann, Jeanette

AU - Hassfeld, Sabine

AU - Kallisch, Heike

AU - Fleck, Eckart

AU - Regitz-Zagrosek, Vera

PY - 1998/3/17

Y1 - 1998/3/17

N2 - To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

AB - To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

UR - https://www.scopus.com/pages/publications/0032539769

U2 - 10.1006/bbrc.1998.8311

DO - 10.1006/bbrc.1998.8311

M3 - Journal articles

C2 - 9514957

AN - SCOPUS:0032539769

SN - 0006-291X

VL - 244

SP - 494

EP - 497

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Abstract

Fördermittel

UN SDGs

Dokumente

Weitere Links

Fingerprint

Zitieren