Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Jeanette Erdmann; Sabine Hassfeld; Heike Kallisch; Eckart Fleck; Vera Regitz-Zagrosek

doi:10.1006/bbrc.1998.8311

Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Jeanette Erdmann^*, Sabine Hassfeld, Heike Kallisch, Eckart Fleck, Vera Regitz-Zagrosek

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Kardiogenetik

Charite - Universitatsmedizin Berlin

9 Zitate (Scopus)

Abstract

To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

Originalsprache	Englisch
Zeitschrift	Biochemical and Biophysical Research Communications
Jahrgang	244
Ausgabenummer	2
Seiten (von - bis)	494-497
Seitenumfang	4
ISSN	0006-291X
DOIs	https://doi.org/10.1006/bbrc.1998.8311
Publikationsstatus	Veröffentlicht - 17.03.1998

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10.1006/bbrc.1998.8311

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abstract = "To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.",

author = "Jeanette Erdmann and Sabine Hassfeld and Heike Kallisch and Eckart Fleck and Vera Regitz-Zagrosek",

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AU - Erdmann, Jeanette

AU - Hassfeld, Sabine

AU - Kallisch, Heike

AU - Fleck, Eckart

AU - Regitz-Zagrosek, Vera

PY - 1998/3/17

Y1 - 1998/3/17

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AB - To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Abstract

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