Characterization of calcium-mobilizing, purinergic P2Y2 receptors in human ovarian cancer cells

Askan Schultze-Mosgau, Ann C. Katzur, Krishan K. Arora, Stanko S. Stojilkovic*, Klaus Diedrich, Olaf Ortmann

*Korrespondierende/r Autor/-in für diese Arbeit
34 Zitate (Scopus)


In human ovarian EFO-21 and EFO-27 carcinoma cells, extracellular ATP induced a concentration-dependent rise in intracellular calcium concentration ([Ca2+](i)), suggesting the expression of a purinoreceptor. ATP and UTP were equipotent in generating [Ca2+](i) signals, followed by ATP-γ-S and ADP, whereas β,γ-ATP, 2 methyl 1 thio-ATP, 3'-o-(4-benzoyl) benzoyl-ATP, AMP, and adenosine were ineffective. This pharmacological profile suggested the presence of the P2Y2 subtype in both cell types, and this was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) analysis using P2Y2 primers. ATP-induced [Ca2+](i) signals were composed of two phases: an early and extracellular calcium-independent phase, followed by a sustained plateau phase that was dependent on capacitative calcium influx. In addition to the rise in the [Ca2+](i), a time- and concentration-dependent increase in phosphatidylethanol accumulation was observed in ATP-stimulated cells, indicating an increase in phospholipase D activity. RT-PCR analysis identified the expression of a transcript for the phospholipase D-1 subtype of this enzyme. Activation of these receptors by a slowly degradable analogue, ATP-γ-S, attenuated basal and fetal calf serum-induced cell proliferation in a time- and concentration-dependent manner. These results indicate that ATP may act as an extracellular messenger in controlling the ovarian epithelial cell cycle through P2Y2 receptors.

ZeitschriftMolecular Human Reproduction
Seiten (von - bis)435-442
PublikationsstatusVeröffentlicht - 2000


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