Characterization of Anaphylatoxin Receptor Expression and C3a/C5a Functions in Anaphylatoxin Receptor Reporter Mice

Yves Laumonnier; Christian M. Karsten; Gabriele Köhl; Jörg Köhl

doi:10.1002/cpim.100

Characterization of Anaphylatoxin Receptor Expression and C3a/C5a Functions in Anaphylatoxin Receptor Reporter Mice

Yves Laumonnier, Christian M. Karsten, Gabriele Köhl, Jörg Köhl^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Systemische Entzündungsforschung

11 Zitate (Scopus)

Abstract

The anaphylatoxins (AT) C3a and C5a are effector molecules of C3 and C5 exerting multiple biologic functions through binding and activation of their cognate G protein−coupled receptors. C3a interacts with the C3a receptor (C3aR), whereas C5a and its primary degradation product C5a-desArg engage C5aR1 and C5aR2. In the past, analysis of AT expression has been hampered by cross reaction of antibodies designed to recognize the different AT receptors. Furthermore, assessment of effects mediated by cell-specific activation has been difficult. Here, floxed AT receptor reporter mice are described as tools to monitor AT receptor expression in cells and tissues and to study the functions of C3a and C5a by cell-specific deletion of their cognate AT receptors.

Originalsprache	Englisch
Aufsatznummer	e100
Zeitschrift	Current Protocols in Immunology
Jahrgang	130
Ausgabenummer	1
ISSN	1934-3671
DOIs	https://doi.org/10.1002/cpim.100
Publikationsstatus	Veröffentlicht - 01.09.2020

Fördermittel

Open access funding enabled and organized by Projekt DEAL. This work was supported by funds from the German Research Foundation (DFG) for the International Research Training Group (IRTG) 1911 (projects A1, A7, B1, and B2 to Y.L., C.M.K., and J.K.), as well as the EXC 306 and EXC 2167 to J.K. We thank Katharina Quell and Katharina Bröker for help in establishing the functional AT receptor assays and T. Vollbrandt for his comments regarding the flow cytometer settings.

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Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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10.1002/cpim.100

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title = "Characterization of Anaphylatoxin Receptor Expression and C3a/C5a Functions in Anaphylatoxin Receptor Reporter Mice",

abstract = "The anaphylatoxins (AT) C3a and C5a are effector molecules of C3 and C5 exerting multiple biologic functions through binding and activation of their cognate G protein−coupled receptors. C3a interacts with the C3a receptor (C3aR), whereas C5a and its primary degradation product C5a-desArg engage C5aR1 and C5aR2. In the past, analysis of AT expression has been hampered by cross reaction of antibodies designed to recognize the different AT receptors. Furthermore, assessment of effects mediated by cell-specific activation has been difficult. Here, floxed AT receptor reporter mice are described as tools to monitor AT receptor expression in cells and tissues and to study the functions of C3a and C5a by cell-specific deletion of their cognate AT receptors.",

author = "Yves Laumonnier and Karsten, \{Christian M.\} and Gabriele K{\"o}hl and J{\"o}rg K{\"o}hl",

note = "Funding Information: Open access funding enabled and organized by Projekt DEAL. This work was supported by funds from the German Research Foundation (DFG) for the International Research Training Group (IRTG) 1911 (projects A1, A7, B1, and B2 to Y.L., C.M.K., and J.K.), as well as the EXC 306 and EXC 2167 to J.K. We thank Katharina Quell and Katharina Br{\"o}ker for help in establishing the functional AT receptor assays and T. Vollbrandt for his comments regarding the flow cytometer settings. Publisher Copyright: {\textcopyright} 2020 The Authors. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.1002/cpim.100",

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AU - Köhl, Gabriele

AU - Köhl, Jörg

N1 - Funding Information: Open access funding enabled and organized by Projekt DEAL. This work was supported by funds from the German Research Foundation (DFG) for the International Research Training Group (IRTG) 1911 (projects A1, A7, B1, and B2 to Y.L., C.M.K., and J.K.), as well as the EXC 306 and EXC 2167 to J.K. We thank Katharina Quell and Katharina Bröker for help in establishing the functional AT receptor assays and T. Vollbrandt for his comments regarding the flow cytometer settings. Publisher Copyright: © 2020 The Authors. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/9/1

Y1 - 2020/9/1

N2 - The anaphylatoxins (AT) C3a and C5a are effector molecules of C3 and C5 exerting multiple biologic functions through binding and activation of their cognate G protein−coupled receptors. C3a interacts with the C3a receptor (C3aR), whereas C5a and its primary degradation product C5a-desArg engage C5aR1 and C5aR2. In the past, analysis of AT expression has been hampered by cross reaction of antibodies designed to recognize the different AT receptors. Furthermore, assessment of effects mediated by cell-specific activation has been difficult. Here, floxed AT receptor reporter mice are described as tools to monitor AT receptor expression in cells and tissues and to study the functions of C3a and C5a by cell-specific deletion of their cognate AT receptors.

AB - The anaphylatoxins (AT) C3a and C5a are effector molecules of C3 and C5 exerting multiple biologic functions through binding and activation of their cognate G protein−coupled receptors. C3a interacts with the C3a receptor (C3aR), whereas C5a and its primary degradation product C5a-desArg engage C5aR1 and C5aR2. In the past, analysis of AT expression has been hampered by cross reaction of antibodies designed to recognize the different AT receptors. Furthermore, assessment of effects mediated by cell-specific activation has been difficult. Here, floxed AT receptor reporter mice are described as tools to monitor AT receptor expression in cells and tissues and to study the functions of C3a and C5a by cell-specific deletion of their cognate AT receptors.

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Characterization of Anaphylatoxin Receptor Expression and C3a/C5a Functions in Anaphylatoxin Receptor Reporter Mice

Abstract

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