Change in Fluorescence Lifetime of Retinal Pigment Epithelium under Oxidative Stress

Objective：Fluorescence lifetime imaging ophthalmoscopy (FLIO) is a new examination method which enabled the measurement of fluorescence lifetime (FLT) of the human fundus. We investigated changes in the FLT of the autofluorescence of retinal pigment epithelium (RPE) in organ culture under oxidative stress. Materials and methods：Ex-vivo porcine RPE was
cultured with hydrogen peroxide(H2 O2)at concentrations of 0, 1.5, and 3 mM and FLIO［excitation wave length：473 nm, detection range：498-560 nm (Channel 1) and 560-720 nm (Channel 2)］was conducted after 24h and 48h. Cell viability and intracellular reactive oxygen species(ROS) were examined using calcein-AM and 2ʼ, 7ʼ-dichlorofluorescein diacetate, respectively.