TY - JOUR
T1 - CDK5RAP2 interaction with components of the Hippo signaling pathway may play a role in primary microcephaly
AU - Sukumaran, Salil K.
AU - Stumpf, Maria
AU - Salamon, Sarah
AU - Ahmad, Ilyas
AU - Bhattacharya, Kurchi
AU - Fischer, Sarah
AU - Müller, Rolf
AU - Altmüller, Janine
AU - Budde, Birgit
AU - Thiele, Holger
AU - Tariq, Muhammad
AU - Malik, Naveed Altaf
AU - Nürnberg, Peter
AU - Baig, Shahid Mahmood
AU - Hussain, Muhammad Sajid
AU - Noegel, Angelika A.
N1 - Publisher Copyright:
© 2016, The Author(s).
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Autosomal recessive primary microcephaly (MCPH) is characterized by a substantial reduction in brain size but with normal architecture. It is often linked to mutations in genes coding for centrosomal proteins; however, their role in brain size regulation is not completely understood. By combining homozygosity mapping and whole-exome sequencing in an MCPH family from Pakistan, we identified a novel mutation (XM_011518861.1; c.4114C > T) in CDK5RAP2, the gene associated with primary microcephaly-3 (MCPH3), leading to a premature stop codon (p.Arg1372*). CDK5RAP2 is a component of the pericentriolar material important for the microtubule-organizing function of the centrosome. Patient-derived primary fibroblasts had strongly decreased CDK5RAP2 amounts, showed centrosomal and nuclear abnormalities and exhibited changes in cell size and migration. We further identified an interaction of CDK5RAP2 with the Hippo pathway components MST1 kinase and the transcriptional regulator TAZ. This finding potentially provides a mechanism through which the Hippo pathway with its roles in the regulation of centrosome number is linked to the centrosome. In the patient fibroblasts, we observed higher levels of TAZ and YAP. However, common target genes of the Hippo pathway were downregulated as compared to the control with the exception of BIRC5 (Survivin), which was significantly upregulated. We propose that the centrosomal deficiencies and the altered cellular properties in the patient fibroblasts can also result from the observed changes in the Hippo pathway components which could thus be relevant for MCPH and play a role in brain size regulation and development.
AB - Autosomal recessive primary microcephaly (MCPH) is characterized by a substantial reduction in brain size but with normal architecture. It is often linked to mutations in genes coding for centrosomal proteins; however, their role in brain size regulation is not completely understood. By combining homozygosity mapping and whole-exome sequencing in an MCPH family from Pakistan, we identified a novel mutation (XM_011518861.1; c.4114C > T) in CDK5RAP2, the gene associated with primary microcephaly-3 (MCPH3), leading to a premature stop codon (p.Arg1372*). CDK5RAP2 is a component of the pericentriolar material important for the microtubule-organizing function of the centrosome. Patient-derived primary fibroblasts had strongly decreased CDK5RAP2 amounts, showed centrosomal and nuclear abnormalities and exhibited changes in cell size and migration. We further identified an interaction of CDK5RAP2 with the Hippo pathway components MST1 kinase and the transcriptional regulator TAZ. This finding potentially provides a mechanism through which the Hippo pathway with its roles in the regulation of centrosome number is linked to the centrosome. In the patient fibroblasts, we observed higher levels of TAZ and YAP. However, common target genes of the Hippo pathway were downregulated as compared to the control with the exception of BIRC5 (Survivin), which was significantly upregulated. We propose that the centrosomal deficiencies and the altered cellular properties in the patient fibroblasts can also result from the observed changes in the Hippo pathway components which could thus be relevant for MCPH and play a role in brain size regulation and development.
UR - http://www.scopus.com/inward/record.url?scp=85006893245&partnerID=8YFLogxK
U2 - 10.1007/s00438-016-1277-x
DO - 10.1007/s00438-016-1277-x
M3 - Journal articles
C2 - 28004182
AN - SCOPUS:85006893245
SN - 1617-4615
VL - 292
SP - 365
EP - 383
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 2
ER -