Abstract
PURPOSE: To examine the viability and differentiation of retinal pigment epithelial (RPE) cells after exposure to the vital dye Acid Violet-17 (AV-17).
METHODS: Bovine RPE cells were incubated with AV-17 (0.0625-0.5 mg/mL) for 30 seconds or 5 minutes. Viability was determined by live/dead staining, cleaved CASP3 immunostainings, and MTT test. Actin cytoskeleton was visualized by Alexa 488-phalloidin. Immunocytochemistry was performed to determine the levels of ZO-1, CTNNB1, and KRT19.
RESULTS: Exposure to AV-17 at the concentrations of 0.25-0.5 mg/mL resulted in a dose-dependent decrease in viability, the loss of ZO-1 from tight junctions, translocation of CTNNB1 into the cytoplasm and nucleus, disarrangement of the actin cytoskeleton, and a slight increase in KRT19.
CONCLUSION: AV-17 at a concentration <0.125 mg/mL is likely to be well tolerated by the RPE cells, whereas the concentrations from 0.25 mg/mL onward can reduce viability and induce dedifferentiation particularly after long-term exposure.
| Titel in Übersetzung | Biokompatibilität des Vitalfarbstoffs Acid Violet-17 auf retinalen Pigmentepithelzellen |
|---|---|
| Originalsprache | Englisch |
| Zeitschrift | Clinical ophthalmology (Auckland, N.Z.) |
| Jahrgang | 10 |
| Seiten (von - bis) | 1435-45 |
| Seitenumfang | 11 |
| ISSN | 1177-5467 |
| DOIs | |
| Publikationsstatus | Veröffentlicht - 2016 |
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