TY - JOUR
T1 - Biglycan expression in the melanoma microenvironment promotes invasiveness via increased tissue stiffness inducing integrin-β1 expression
AU - Andrlová, Hana
AU - Mastroianni, Justin
AU - Madl, Josef
AU - Kern, Johannes S.
AU - Melchinger, Wolfgang
AU - Dierbach, Heide
AU - Wernet, Florian
AU - Follo, Marie
AU - Hafsi, Kristin Technau
AU - Has, Cristina
AU - Mittapalli, Venugopal Rao
AU - Idzko, Marco
AU - Herr, Ricarda
AU - Brummer, Tilman
AU - Ungefroren, Hendrik
AU - Busch, Hauke
AU - Boerries, Melanie
AU - Narr, Andreas
AU - Ihorst, Gabriele
AU - Vennin, Claire
AU - Schmitt-Graeff, Annette
AU - Minguet, Susana
AU - Timpson, Paul
AU - Duyster, Justus
AU - Meiss, Frank
AU - Römer, Winfried
AU - Zeiser, Robert
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Novel targeted and immunotherapeutic approaches have revolutionized the treatment of metastatic melanoma. A better understanding of the melanomamicroenvironment, in particular the interaction of cells with extracellular matrix molecules, may help to further improve these new therapeutic strategies. We observed that the extracellular matrix molecule biglycan (Bgn) was expressed in certain human melanoma cells and primary fibroblasts when evaluated by microarraybased gene expression analysis. Bgn expression in the melanoma tissues correlated with low overall-survival and low progression-free-survival in patients. To understand the functional role of Bgn we used gene-targeted mice lacking functional Bgn. Here we observed that melanoma growth, metastasis-formation and tumor-related death were reduced in Bgn-/- mice compared to Bgn+/+ mice. In vitro invasion of melanoma cells into organotypic-matrices derived from Bgn-/- fibroblasts was reduced compared to melanoma invasion into Bgn-proficient matrices. Tissue stiffness as determined by atomic-force-microscopy was reduced in Bgn-/- matrices. Isolation of melanoma cells and fibroblasts from the stiffer Bgn+/+ matrices revealed an increase in integrin-β1 expression compared to the Bgn-/- fibroblast matrices. Overexpression of integrin-β1 in B16-melanoma cells abolished the survival benefit seen in Bgn-/- mice. Consistent with the studies performed in mice, the abundance of Bgn-expression in human melanoma samples positively correlated with the expression of integrin-β1, which is in agreement with results from the organotypic invasion-assay and the in vivo mouse studies. This study describes a novel role for Bgn-related tissue stiffness in the melanomamicroenvironment via regulation of integrin-β1 expression by melanoma cells in both mice and humans.
AB - Novel targeted and immunotherapeutic approaches have revolutionized the treatment of metastatic melanoma. A better understanding of the melanomamicroenvironment, in particular the interaction of cells with extracellular matrix molecules, may help to further improve these new therapeutic strategies. We observed that the extracellular matrix molecule biglycan (Bgn) was expressed in certain human melanoma cells and primary fibroblasts when evaluated by microarraybased gene expression analysis. Bgn expression in the melanoma tissues correlated with low overall-survival and low progression-free-survival in patients. To understand the functional role of Bgn we used gene-targeted mice lacking functional Bgn. Here we observed that melanoma growth, metastasis-formation and tumor-related death were reduced in Bgn-/- mice compared to Bgn+/+ mice. In vitro invasion of melanoma cells into organotypic-matrices derived from Bgn-/- fibroblasts was reduced compared to melanoma invasion into Bgn-proficient matrices. Tissue stiffness as determined by atomic-force-microscopy was reduced in Bgn-/- matrices. Isolation of melanoma cells and fibroblasts from the stiffer Bgn+/+ matrices revealed an increase in integrin-β1 expression compared to the Bgn-/- fibroblast matrices. Overexpression of integrin-β1 in B16-melanoma cells abolished the survival benefit seen in Bgn-/- mice. Consistent with the studies performed in mice, the abundance of Bgn-expression in human melanoma samples positively correlated with the expression of integrin-β1, which is in agreement with results from the organotypic invasion-assay and the in vivo mouse studies. This study describes a novel role for Bgn-related tissue stiffness in the melanomamicroenvironment via regulation of integrin-β1 expression by melanoma cells in both mice and humans.
UR - http://www.scopus.com/inward/record.url?scp=85021273777&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.17160
DO - 10.18632/oncotarget.17160
M3 - Journal articles
AN - SCOPUS:85021273777
SN - 1949-2553
VL - 8
SP - 42901
EP - 42916
JO - Oncotarget
JF - Oncotarget
IS - 26
ER -