Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix

Rudolf Manz, Mario Assenmacher, Eckhard Pflüger, Stefan Miltenyi, Andreas Radbruch

237 Zitate (Scopus)

Abstract

We have developed a technology for analysis and sorting of live cells according to secreted molecules. An artificial affinity matrix, specific for the secreted product of interest, is created on the cell surface, and the cells are allowed to secrete for a defined time period. The secreted molecules bind to the affinity matrix on the secreting cell and are subsequently labeled with specific fluorescent or magnetic staining reagents for cytometric analysis and cell sorting. Crossfeeding of the secreted products to other cells is prevented by decreasing the permeability of the incubation medium. This approach will have a wide range of applications in biotechnology and biomedical research. Here, we describe analysis and sorting of hybridoma cells, according to secreted antibodies, and of activated T lymphocytes, according to secreted cytokines.

OriginalspracheEnglisch
ZeitschriftProceedings of the National Academy of Sciences of the United States of America
Jahrgang92
Ausgabenummer6
Seiten (von - bis)1921-1925
Seitenumfang5
ISSN0027-8424
DOIs
PublikationsstatusVeröffentlicht - 14.03.1995

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

Fingerprint

Untersuchen Sie die Forschungsthemen von „Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix“. Zusammen bilden sie einen einzigartigen Fingerprint.

Zitieren